마디풀 추출물의 경피 전달을 위한 탄성 리포좀의 물리적 특성 및 In Vitro 피부 투과 연구

한샛별; 권순식; 정유민; 공봉주; 유은령; 박수남; Saet Byeol Han; Soon Sik Kwon; Yoo Min Jeong; Bong Ju Kong; Eun Ryeong Yu; Soo Nam Park

Polymer(Korea), Vol.38, No.6, 694-701, November, 2014

Physical Characterizations and In Vitro Skin Permeation of Elastic Liposomes for Transdermal Delivery of Polygonum aviculare L. Extract

한샛별, 권순식, 정유민, 공봉주, 유은령, 박수남

서울과학기술대학교 정밀화학과 나노바이오 화장품연구실, 화장품종합기술연구소

Saet Byeol Han, Soon Sik Kwon, Yoo Min Jeong, Bong Ju Kong, Eun Ryeong Yu, and Soo Nam Park

Department of Fine Chemistry, Nanobiocosmetic Laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul 139-743, Korea

초록

본 연구에서는 다양한 플라보노이드를 주성분으로 하는 마디풀 추출물의 피부 흡수를 증진시키기 위하여 인지질(PC)과 계면활성제(Tego® care 450)로 구성된 탄성 리포좀(ELPs)을 제조하였으며, 그것의 물리적 특성 및 invitro 피부 투과능을 평가하였다. 마디풀 추출물을 담지한 탄성 리포좀의 평균 입자 크기는 148.1~262.2 nm, 가변형성 지수는 11.5~25.4, 포집 효율은 53.1~66.3%로 나타났다. Franz diffusion cell을 이용한 in vitro 피부 투과 실험결과, 인지질 대 계면활성제 비율이 85:15인 ELP-4가 계면활성제가 포함되지 않은 일반 리포좀인 ELP-1보다 더 높은 피부 투과능을 갖는 것을 확인하였으며, 이를 형광 이미지 복원 현미경을 통해 가시적으로 확인하였다. 결론적으로 최적 조건으로 선정된 ELP-4는 마디풀 추출물의 피부 흡수 증진을 위한 유용한 피부 전달체로 이용될 수 있음을 확인하였다.

In this study, Polygomun aviculare L. (P. aviculare L.) extract loaded elastic liposomes (ELPs) were investigated to enhance the transdermal delivery of P. aviculare L. extract composed of various flavonoids. ELPs were composed of egg phospholipids (PC) and edge activator (Tego® care 450) and the physical properties and in vitro permeation studies of ELPs were performed. Particle size ranged from 148.1 to 262.2 nm and deformability index was recorded as 11.5~25.4. Loading efficiency was from 53.1 to 66.3%. In vitro skin permeation studies using Franz diffusion cell demonstrated that ELP-4 having ratio of 85:15 for PC to Tego® care 450 exhibited the higher skin permeability than ELP-1, the general liposome without Tego® care 450. It was visually seen by fluorescence image restoration microscopy. The findings suggest that ELP-4 selected as the optimal formulation could be used as useful formulation for transdermal delivery of the extract.

Keywords:elastic liposome;Polygonum aviculare L. extract;Tego® care 450;Franz diffusion cell;skin permeation.

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Lopez O, Maza ADL, Coderch L, Lopez-Iglesias C, Wehrli E, Parra JL, FEBS, 426, 314 (1998)
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Singh AK, Cummings EB, Throckmorton DJ, Anal. Chem., 73, 1057 (2001)

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[2] Yaar M, Gilchrest BA, Br. J. Dermatol., 157, 874 (2007)

[3] Won DH, Han SB, Hwang JP, Kim SJ, Park JN, Park SN, J. Soc. Cosmet. Scientists Korea, 38, 297 (2012)

[4] Bouwstra JA, Honeywell-Nguyen PL, Adv. Drug Deliv. Rev., 54, 41 (2002)

[5] Honeywell-Nguyen PL, Bouwstra JA, Drug Delivery/Formulation and Nanotechnology, 2, 67 (2005)

[6] Lim MS, Han SB, Kwon SS, Park MA, Park SN, J. Soc. Cosmet. Scientists Korea, 38, 147 (2012)

[7] Sharma A, Sharma US, Int. J. Pharm., 154, 123 (1997)

[8] Choi MJ, Mailbach HI, Int. J. Cosmet. Sci., 27, 211 (2005)

[9] Elsayed MMA, Abdallah OY, Naggar VF, Khalafallah NM, Int. J. Pharm., 332, 1 (2007)

[10] Harsh V, Annu K, Rahul B, Vandana J, Int. Res. J. Pharm., 3, 117 (2012)

[11] Yunuskhodzhaeva NA, Eshbakova KA, Abdullabekova VN, Chem. Nat. Compd., 46, 803 (2010)

[12] Jeon CB, Dissertation MS, Graduate School of Education Chungbuk National University, Korea (2012)

[13] Lee CG, Kim NJ, Hong ND, Kwon CH, Kor. J. Pharmacogn., 25, 59 (1994)

[14] Kim MJ, Dissertation MS, Graduate School of Seoul National University of Science and Technology, Korea (2012)

[15] Nijveldt RJ, Nood EV, Hoorn DECV, Boelens PG, Norren KV, Leeuwen PAMV, Am. J. Clin. Nutr., 74, 418 (2001)

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[17] Park SN, Lim MS, Park MA, Kwon SS, Han SB, Polym.(Korea), 36(6), 705 (2012)

[18] Lopez O, Maza ADL, Coderch L, Lopez-Iglesias C, Wehrli E, Parra JL, FEBS, 426, 314 (1998)

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[20] Singh AK, Cummings EB, Throckmorton DJ, Anal. Chem., 73, 1057 (2001)