Fabrication and characterization of electrospun curcumin-loaded polycaprolactone-polyethylene glycol nanofibers for enhanced wound healing

Hieu Trung Bui; Ok Hee Chung; Joseph Dela Cruz; Jun Seo Park

Macromolecular Research, Vol.22, No.12, 1288-1296, December, 2014

DOI10.1007/s13233-014-2179-6 Export Citation

Fabrication and characterization of electrospun curcumin-loaded polycaprolactone-polyethylene glycol nanofibers for enhanced wound healing

Hieu Trung Bui, Ok Hee Chung¹, Joseph Dela Cruz², and Jun Seo Park^†

Department of Chemical Engineering and Research Center of Chemical Technology, Hankyong National University, Gyeonggi, 456-749, Korea
¹Department of Physics, Sunchon National University, Chunnam, 540-950, Korea
²College of Veterinary Medicine University of the Philippines Los Banos, Manila, Philippines

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This study focused on the development of biomedicated electrospun nanofiber mats for preventing wound infections and accelerating wound healing. Polycaprolactone (PCL) nanofibers-loaded curcumin (Cur) and polyethylene glycol (PEG) were generated by an electrospinning technique. The change in surface morphology of the electrospun nanofibers to porous surface after immersion was obtained by field emission scanning electron microscopy (FE-SEM). The biological characteristics of the Cur-loaded PCL-PEG nanofiber mats such as cell viability, cell attachment, anti-inflammatory and antibacterial properties, and in vivo wound healing capability were examined. The blending of PEG with PCL resulted in the formation of pores on the nanofibers after immersion, which supports cell viability and proliferation. The mouse myoblast cell line C2C12 showed about 80% viability on the Cur-loaded PCL-PEG nanofiber mat. SEM images showed that the cells could extremely attach and spread out over the surface of the Cur-loaded PCL-PEG nanofiber mat. The inclusion of 0.5 wt% Cur (with respect to PCL) in both the PCL and PCL-PEG blended nanofiber mats inhibited excessive production of nitric oxide (NO) in RAW264.7 mouse macrophages and exhibited good antibacterial activity against Staphylococcus aureus (S. aureus). In vivo wound healing showed that the treatment using Cur-loaded PCL-PEG nanofiber mat significantly increased the rate of wound closure (99%) on day 10 as compared that using PCL nanofiber mat (59%). These results suggest that the PCL nanofiber matrix containing Cur and PEG can facilitate wound healing with cell proliferation and anti-inflammatory properties.

Keywords:polycaprolactone;curcumin;polyethylene glycol;electrospinning;wound dressing

[References]

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[2] Queen D, Orsted H, Sanada H, Sussman G, Int. Wound J., 1, 59 (2004)

[3] Jannesari M, Varshosaz J, Morshed M, Zamani M, Int. J. Nanomedicine, 6, 993 (2011)

[4] Rutten HJ, Nijhuis PH, Eur. J. Surg. Suppl., 31 (1997)

[5] El-Sherif H, El-Masry M, Kansoh A, Macromol. Res., 19(11), 1157 (2011)

[6] Sikareepaisan P, Ruktanonchai U, Supaphol P, Carbohydr. Polym., 83, 1457 (2011)

[7] Kim MS, Hong KD, Shin HW, Kim SH, Kim SH, Lee MS, Jang WY, Khang G, Lee HB, Int. J. Biol. Macromol., 36, 54 (2005)

[8] Al-Youssef HM, Amina M, Hassan S, Amna T, Jeong JW, Nam KT, Kim HY, Macromol. Res., 21(6), 589 (2013)

[9] Zeng J, Xu X, Chen X, Liang Q, Bian X, Yang L, Jing X, J. Control. Release, 92, 227 (2003)

[10] Lee JS, Kim JK, Park SR, Chang YH, Macromol. Res., ., 15(3), 205 (2007)

[11] Nguyen TTT, Ghosh C, Hwang SG, Tran LD, Park JS, J. Mater. Sci., 48(20), 7125 (2013)

[12] Pant HR, Neupane MP, Pant B, Panthi G, Oh HJ, Lee MH, Kim HY, Colloids Surf. B: Biointerfaces, 88, 587 (2011)

[13] Xu X, Yang L, Xu X, Wang X, Chen X, Liang Q, Zeng J, Jing X, J. Control. Release, 108, 33 (2005)

[14] Khil MS, Cha DI, Kim HY, Kim IS, Bhattarai N, J. Biomed. Mater. Res. B: Appl. Biomater., 67, 675 (2003)

[15] Ko JH, Yin H, An J, Chung DJ, Kim JH, Lee SB, Pyun DG, Macromol. Res., 18(2), 137 (2010)

[16] Au HT, Pham LN, Thi Vu TH, Park JS, Macromol. Res., 20(1), 51 (2012)

[17] Luong-Van E, Grøndahl L, Chua KN, Leong KW, Nurcombe V, Cool SM, Biomaterials, 27, 2042 (2006)

[18] Dash TK, Konkimalla VB, J. Control. Release, 158, 15 (2012)

[19] Merrell JG, McLaughlin SW, Tie L, Laurencin CT, Chen AF, Nair LS, Clin. Exp. Pharmacol. Physiol., 36, 1149 (2009)

[20] Han SO, Son WK, Youk JH, Lee TS, Park WH, Mater. Lett., 59, 2998 (2005)

[21] Nguyen TT, Ghosh C, Hwang SG, Chanunpanich N, Park JS, Int. J. Pharm., 439, 296 (2012)

[22] Zhang YZ, Feng Y, Huang ZM, Ramakrishna S, Lim CT, Nanotechnology, 17, 901 (2006)

[23] Thangapazham RL, Sharma A, Maheshwari RK, Adv. Exp. Med. Biol., 595, 343 (2007)

[24] Yang KYL, Lei-Chwen, Tseng TY, Wang SC, Tsai TH, J. Chromatogr. B, 853, 183 (2007)

[25] Jurenka JS, Altern. Med. Rev., 14, 141 (2009)

[26] Panchatcharam M, Miriyala S, Gayathri V, Suguna L, Mol. Cell. Biochem., 290, 87 (2006)

[27] Sidhu GS, Singh AK, Thaloor D, Banaudha KK, Patnaik GK, Srimal RC, Maheshwari RK, Wound Repair Regen., 6, 167 (1998)

[28] Gopinath D, Ahmed MR, Gomathi K, Chitra K, Sehgal PK, Jayakumar R, Biomaterials, 25, 1911 (2004)

[29] Wang X, Jiang Y, Wang YW, Huang MT, Ho CT, Huang Q, Food Chem., 108, 419 (2008)

[30] Gou M, Men K, Shi H, Xiang M, Zhang J, Song J, Long J, Wan Y, Luo F, Zhao X, Qian Z, Nanoscale, 3, 1558 (2011)

[31] Ratanajiajaroen P, Watthanaphanit A, Tamura H, Tokura S, Rujiravanit R, Eur. Polym. J., 48, 512 (2012)

[32] Suwantong O, Opanasopit P, Ruktanonchal U, Supaphol P, Polymer, 48(26), 7546 (2007)

[33] Nguyen TTT, Chung OH, Park JS, Carbohydr. Polym., 86, 1799 (2011)

[34] Hsu CM, Shivkumar S, Macromol. Mater. Eng., 289, 334 (2004)

[35] Zeng J, Yang L, Liang Q, Zhang X, Guan H, Xu X, Chen X, Jing X, J. Control. Release, 105, 43 (2005)

[36] Sung JH, Hwang MR, Kim JO, Lee JH, Kim YI, Kim JH, Chang SW, Jin SG, Kim JA, Lyoo WS, Han SS, Ku SK, Yong CS, Choi HG, Int. J. Pharm., 392, 232 (2010)

[37] Notoya M, Nishimura H, Woo JT, Nagai K, Ishihara Y, Hagiwara H, Eur. J. Pharmacol., 534, 55 (2006)

[38] Scharstuhl A, Mutsaers HA, Pennings SW, Szarek WA, Russel FG, Wagener FA, J. Cell. Mol. Med., 13, 712 (2009)

[39] Rao YK, Fang SH, Tzeng YM, J. Ethnopharmacol., 114, 78 (2007)

[40] Dunphy JE, Edwards LC, Udupa KN, Ann. Surg., 144, 304 (1956)