스트론튬(Strontium)이 도핑된 다공성 BCP 뼈 이식제가 조골세포에 미치는 영향

변인선; Swapan Kumar Sarkar; 서형석; 이병택; 송호연; In-Seon Byun; Swapan Kumar Sarkar; Hyung-Seok Seo; Byong-Taek Lee; Ho-Yeon Song

Korean Journal of Materials Research, Vol.20, No.3, 155-160, March, 2010

DOI10.3740/MRSK.2010.20.3.155 Export Citation

스트론튬(Strontium)이 도핑된 다공성 BCP 뼈 이식제가 조골세포에 미치는 영향

Effect of Strontium Doped Porous BCP as Bone Graft Substitutes on Osteoblast

변인선, Swapan Kumar Sarkar¹, 서형석², 이병택¹, 송호연^†

순천향대학교 의과대학 면역학교실
¹의공학교실
²건양대학교 운동처방학과

In-Seon Byun, Swapan Kumar Sarkar¹, Hyung-Seok Seo², Byong-Taek Lee¹, and Ho-Yeon Song^†

Dept. of Immunology, Biomedical engineering, Korea
¹Medical School, SoonChunHyang University, 366-1, Ssangyong-dong, Cheonan, Chungnam, Korea
²Dept. of Exercise Prescription, Konyang, Konyang University, 119, Daehakro, Nonsan, Chungnam, Korea

E-mail:

In this study, we investigated primary biocompatibility and osteogenic gene expression of porous granular BCP bone substitutes with or without strontium (Sr) doping. In vitro biocompatibility was investigated on fibroblasts like L929 cells and osteoblasts like MG-63 cells using a cell viability assay (MTT) and one cell morphological observation by SEM, respectively. MTT results showed a cell viability percent of L929 fibroblasts, which was higher in Sr-BCP granules (98-101%) than in the non-doped granules (92-96%, p < 0.05). Osteoblasts like MG-63 cells were also found to proliferate better on Sr-doped BCP granules (01-111%) than on the non-doped ones (92-99%, p < 0.05) using an MTT assay. As compared with pure BCP granules, SEM images of MG-63 cells grown on sample surfaces confirmed that cellular spreading, adhesion and proliferation were facilitated by Sr doping on BCP. Active filopodial growth of MG-63 cells was also observed on Srdoped BCP granules. The cells on Sr-doped BCP granules were well attached and spread out. Gene expression of osteonectin, osteopontin and osteoprotegrin were also evaluated using reverse transcriptase polymerase chain reaction (RTPCR), which showed that the mRNA phenotypes of these genes were well maintained and expressed in Sr-doped BCP granules. These results suggest that Sr doping in a porous BCP granule can potentially enhance the biocompatibility and bone ingrowth capability of BCP biomaterials.

Keywords:BCP;strontium doping;porous bone substitute;biocompatibility

[References]

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Okumura A, Goto M, Goto T, Yoshinari M, Masuko S, Katsuki T, Tanaka T, Biomaterials, 22, 2263 (2001)
Puleo DA, Nanci A, Biomaterials, 20, 2311 (1999)
Anselme K, Biomaterials, 21(7), 667 (2000)
Canalis E, Hott M, Deloffre P, Tsouderos Y, Marie PJ, Bone, 18(6), 517 (1996)
Chattopadhyay N, Quinn SJ, Kifor O, Ye C, Brown EM, Biochem. Pharmacol., 74(3), 438 (2007)
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Grynpas MD, Marie PJ, Bone, 11(5), 313 (1990)
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Steven CV, DeB Marc E, Patrick CD, Kidney Int., 64, 534 (2003)
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Zhu LL, Zaidi S, Peng YZ, Zhou H, Moonga BS, Blesius A, Dupin-Roger I, Zaidi M, Sun L, Biochem. Biophys. Res. Commun., 355(2), 307 (2007)
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Webb K, Hlady V, Tresco PA, J. Biomed. Mater. Res., 49(3), 362 (2000)
Hott M, Noel B, Bernache-Assolant D, Rey C, Marie PJ, J. Biomed. Mater. Res., 37, 508 (1997)
Pioletti DP, Muller J, Rakotomanana LR, Biomechanics, 36, 131 (2003)
Bigerelle M, Anselme K, Dufresne E, Biomol. Eng., 19, 79 (2002)
Rajaraman R, Rounds DE, Yen SP, Rembaum A, Exp. Cell Res., 88, 327 (1974)
Schakenraad JM, Buscher HJ, Wildevuur CR, Arends J, J. Biomed. Mater. Res., 20, 773 (1986)
Stein GS, Lian JB, Endocr. Rev., 14(4), 424 (1993)
Choi JY, Lee BH, Song KB, Park RW, Kim IS, Sohn KY, Jo JS, Ryoo HM, J. Cell Biochem., 61(4), 609 (1996)
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[Referenced By]

[1] Schwarz A, Hoffmann F, L'age-Stehr J, Teqzess AM, Transplantation, 44(1), 21 (1987)

[2] Crange A, Gray F, Cesaro P, Adle-Biassette H, Duvoux C, Bell J, Parchi P, Gambetti P, Degos JD, Ann. Neuro., 38(2), 269 (1995)

[3] Gauthier O, Goyenvalle E, Bouler JM, Guicheux J, Pilet P, Weiss P, Daculsi G, J. Mater. Sci. Mater. Med., 12(5), 385 (2001)

[4] Yuan H, Yang Z, Bruijn JD, De Groot K, Zhang X, Biomaterials, 22(19), 2617 (2001)

[5] Anselme K, Osteoblast adhesion on biomaterials, Biomaterials, 21, 667 (2000). (2000)

[6] Okumura A, Goto M, Goto T, Yoshinari M, Masuko S, Katsuki T, Tanaka T, Biomaterials, 22, 2263 (2001)

[7] Puleo DA, Nanci A, Biomaterials, 20, 2311 (1999)

[8] Anselme K, Biomaterials, 21(7), 667 (2000)

[9] Canalis E, Hott M, Deloffre P, Tsouderos Y, Marie PJ, Bone, 18(6), 517 (1996)

[10] Chattopadhyay N, Quinn SJ, Kifor O, Ye C, Brown EM, Biochem. Pharmacol., 74(3), 438 (2007)

[11] Bonnelye E, Chabadel A, Saltel F, Jurdic P, Bone, 42(1), 129 (2008)

[12] Grynpas MD, Marie PJ, Bone, 11(5), 313 (1990)

[13] BarbaraA, Delannoy P, Denis BG, Marie PJ, Metabolism, 53(4), 532 (2004)

[14] Morohashi T, Sano T, Yamada S, Jpn. J. Pharmacol., 64(3), 155 (1994)

[15] Steven CV, DeB Marc E, Patrick CD, Kidney Int., 64, 534 (2003)

[16] Ammann P, Shen V, Robin B, Mauras Y, Bonjour JP, Rizzole R, J. Bone Miner. Res., 19(12), 2012 (2004)

[17] Tournis S, J. Musculoskelet Neuronal Interact, 7(3), 266 (2007)

[18] Zhu LL, Zaidi S, Peng YZ, Zhou H, Moonga BS, Blesius A, Dupin-Roger I, Zaidi M, Sun L, Biochem. Biophys. Res. Commun., 355(2), 307 (2007)

[19] Choudhary S, Wadhwa S, Raisz LG, Alander C, Pibeam CC, J. Bone Miner. Res., 18(10), 1813 (2003)

[20] Gain AK, Lee BT, Materials Science and Engineering: A, 419, 269 (2006)

[21] Lee BT, Kang IC, Gain AK, Kim KH, Song HY, J. of the European Ceramic Society, 26, 3525 (2006)

[22] Primer3 (v. 0.4.0) Pick primers from a DNA sequence, http://frodo.wi.mit.edu/primer3. Retrieved July 22, 2009. (2009)

[23] Nanci A, Wuest JD, Peru L, Brunet P, Sharma V, Zalzal S, McKee MD, J. Biomed. Mater. Res., 40, 324 (1998)

[24] Webb K, Hlady V, Tresco PA, J. Biomed. Mater. Res., 49(3), 362 (2000)

[25] Hott M, Noel B, Bernache-Assolant D, Rey C, Marie PJ, J. Biomed. Mater. Res., 37, 508 (1997)

[26] Pioletti DP, Muller J, Rakotomanana LR, Biomechanics, 36, 131 (2003)

[27] Bigerelle M, Anselme K, Dufresne E, Biomol. Eng., 19, 79 (2002)

[28] Rajaraman R, Rounds DE, Yen SP, Rembaum A, Exp. Cell Res., 88, 327 (1974)

[29] Schakenraad JM, Buscher HJ, Wildevuur CR, Arends J, J. Biomed. Mater. Res., 20, 773 (1986)

[30] Stein GS, Lian JB, Endocr. Rev., 14(4), 424 (1993)

[31] Choi JY, Lee BH, Song KB, Park RW, Kim IS, Sohn KY, Jo JS, Ryoo HM, J. Cell Biochem., 61(4), 609 (1996)

[32] Giachelli CM, Steitz S, Matrix Biology, 19, 615 (2000)