Enzyme-Conjugated CdSe/ZnS Quantum Dot Biosensors for Glucose Detection

Gang-Il Kim; Yun-Mo Sung

Korean Journal of Materials Research, Vol.19, No.1, 44-49, January, 2009

DOI10.3740/MRSK.2009.19.1.044 Export Citation

Enzyme-Conjugated CdSe/ZnS Quantum Dot Biosensors for Glucose Detection

Gang-Il Kim, and Yun-Mo Sung^†

Department of Materials Science and Engineering, Korea University, Seoul 136-713, Korea

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Conjugated nanocrystals using CdSe/ZnS core/shell nanocrystal quantum dots modified by organic linkers and glucose oxidase (GOx) were prepared for use as biosensors. The trioctylphophine oxide (TOPO)-capped QDs were first modified to give them water-solubility by terminal carboxyl groups that were bonded to the amino groups of GOx through an EDC/NHS coupling reaction. As the glucose concentration increased, the photoluminescence intensity was enhanced linearly due to the electron transfer during the enzymatic reaction. The UV-visible spectra of the as-prepared QDs are identical to that of QDs-MAA. This shows that these QDs do not become agglomerated during ligand exchanges. A photoluminescence (PL) spectroscopic study showed that the PL intensity of the QDs-GOx bioconjugates was increased in the presence of glucose. These glucose sensors showed linearity up to approximately 15 mM and became gradually saturated above 15 mM because the excess glucose did not affect the enzymatic oxidation reaction past that amount. These biosensors show highly sensitive variation in terms of their photoluminescence depending on the glucose concentration.

Keywords:CdSe/ZnS quantum dots;ligand exchange;glucose oxidase(GOx);photoluminescense(PL)

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[2] Alivisatos AP, Science, 271(5251), 933 (1996)

[3] Murray CB, Norris DJ, Bawendi MG, J. Am. Chem. Soc., 115, 8706 (1993)

[4] Sung YM, Lee YJ, Park KS, J. Am. Chem. Soc., 128(28), 9002 (2006)

[5] Dabbousi BO, Rodriguezviejo J, Mikulec FV, Heine JR, Mattoussi H, Ober R, Jensen KF, Bawendi MG, J. Phys. Chem. B, 101(46), 9463 (1997)

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[9] Liu H, Hu N, Electroanal., 19, 884 (2007)

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[12] Sung YM, Park KS, Lee YJ, Chae WS, Song YW, Electrochem. Solid State Lett., 8(2), H24 (2005)

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[17] Chan WCW, Nie SM, Science, 281(5385), 2016 (1998)

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[20] Patel N, Davies MC, Hartshorne M, Heaton RJ, Roberts CJ, Tendler SJ, Williams PM, Langmuir, 13(24), 6485 (1997)

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