Investigation the activity and stability of lysozyme on presence of magnetic nanoparticles

B. Shareghi; S. Farhadian; N. Zamani; M. Salavati-Niasari; H. Moshtaghi; S. Gholamrezaei

Journal of Industrial and Engineering Chemistry, Vol.21, 862-867, January, 2015

DOI10.1016/j.jiec.2014.04.024 Export Citation

Investigation the activity and stability of lysozyme on presence of magnetic nanoparticles

B. Shareghi, S. Farhadian, N. Zamani, M. Salavati-Niasari^{1, †}, H. Moshtaghi, and S. Gholamrezaei¹

Department of Biology, Faculty of Science, University of Shahrekord, P.O. Box 115, Shahrekord, Islamic Republic of Iran
¹Institute of Nano Science and Nano Technology, University of Kashan, P.O. Box 87317-51167, Kashan, Islamic Republic of Iran

E-mail:

In this work the influence of Fe2O3 magnetic nanoparticle on lysozyme activity and thermal stability were investigated. The effect of nano-Fe2O3 on lysozyme activity was studied by UV-vis spectrophotometry at 35 ℃ in pH = 7.25 using sodium phosphate as a buffer. Measurements were carried out using 6 × 10^(-8) M (0.1 mg/ml) of lysozyme and a range of nano-Fe2O3 concentrations between 0 and 0.045 mg/ml. It was found that by increasing the concentration of nano-Fe2O3, the rate of Micrococcus lysodeikticus lyses (lysozyme activity) will be increased. On the other hand, the thermal stability of lysozyme was investigated in the presence of nano-Fe2O3 over the temperature range (from 293 K to 363 K). Results indicate that by increasing of nano-Fe2O3, the thermal stability of lysozyme will be increased.

Keywords:Lysozyme;Magnetic nanoparticle;Spectrophotometry;Protein

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[Referenced By]

[1] Hund-Rinke K, Simon M, Environ. Sci. Pollut. Res., 13, 225 (2006)

[2] Stone V, Johnston H, Clift MJD, Geosci. Remote Sens. IEEE Trans., 6, 331 (2007)

[3] Wang Y, Wang B, Zhu MT, Li M, Wang HJ, Wang M, Ouyang H, Chai ZF, Feng WY, Zhao YL, Toxicol. Lett., 205, 26 (2011)

[4] Wang JJ, Sanderson BJS, Wang H, Mutat. Res.: Genet. Toxicol. Environ., 628, 99 (2007)

[5] Lovern SB, Strickler JR, Klaper R, Environ. Sci. Technol., 41, 4465 (2007)

[6] Lu NH, Zhu ZN, Zhao XQ, Tao R, Yang XL, Gao ZH, Biochem. Biophys. Res. Commun., 370(4), 675 (2008)

[7] Chen XH, Zhang L, Weng YX, Du LC, Ye MP, Yang GZ, Fujii R, Rondonuwu FS, Koyama Y, Wu YS, Biophys. J., 88, 4262 (2005)

[8] Oberd G, Oberd E, Oberd J, Environ. Health Perspect., 113, 823 (2005)

[9] Stern ST, McNeil SE, Toxicol. Sci., 101, 4 (2008)

[10] Buzea C, Pacheco I, Robbie K, Comp. Biochem. Physiol., 143, 123 (2006)

[11] Heinlaan M, Ivask A, Blinova I, Dubourguier HC, Kahru A, Chemosphere, 71, 1308 (2008)

[12] Kreyling WG, Semmler M, Erbe F, Mayer P, Takenaka S, Schulz H, Oberd G, Ziesenis A, J. Toxicol. Environ. Health Part A, 65, 1513 (2002)

[13] Elder A, Gelein R, Silva V, Feikert T, Opanashuk L, Carter J, Potter R, Maynard A, Ito Y, Finkelstein J, Environ. Health Perspect., 114, 1172 (2006)

[14] Lockman PR, Oyewumi MO, Koziara JM, Roder KE, Mumper RJ, Allen DD, J. Control. Release, 93, 271 (2003)

[15] Elbaz A, Levecque C, Clavel J, Vidal JS, Richard F, Amouyel P, Alp A, Chartieran MC, Tzourio C, Ann. Neurol., 55, 430 (2004)

[16] Bellova A, Bystrenova E, Koneracka M, Kopcansky P, Valle F, Tomasovicova N, Timko M, Bagelova J, Biscarini F, Gazova Z, Nanotechnology, 21, 1 (2010)

[17] Skaat H, Margel S, Biochem. Biophys. Res. Commun., 386(4), 645 (2009)

[18] Shamim N, Liang H, Hidajat K, Uddin MS, J. Colloid Interface Sci., 320(1), 15 (2008)

[19] Peng ZG, Hidajat K, Uddin MS, Colloids Surf. B: Biointerfaces, 35, 169 (2004)

[20] Sun J, Xu R, Yang Y, J. Chromatogr. B, 879, 3053 (2011)

[21] Blake CCF, Koenig DF, Mair GA, North ACT, Phillips DC, Sarma VR, Nature, 206, 757 (1965)

[22] Cheetham JC, Artymiuk OJ, Phillips DC, J. Mol. Biol., 224, 613 (1992)

[23] Johnson LN, Phillips DC, Nature, 206, 761 (1965)

[24] Murzin AG, Brenner SE, Hubbard T, Chothia C, J. Mol. Biol., 247, 536 (1995)

[25] Bai P, Peng Z, J. Mol. Biol., 314, 321 (2001)

[26] Fischer B, Experientia, 75, 143 (1996)

[27] Tachibana H, Oka T, Akasaka K, J. Mol. Biol., 314, 311 (2001)

[28] Shareghi B, Farhadian S, Salavati-Niasarib M, J. Nanostruct., 1, 205 (2012)

[29] Farhadian S, Shareghi B, Salavati-Niasari M, Amooaghaeia R, J. Nanostruct., 1, 95 (2011)

[30] Gharagozlou M, Boghaei DM, Spectroc. Acta Pt. A-Molec. Biomolec. Spectr., 71, 1621 (2008)

[31] Fleming A, Proc. R. Soc. B: Biol. Sci., 93, 306 (1922)

[32] Wu YL, He F, He XW, Li WY, Zhang YK, Spectroc. Acta Pt. A-Molec. Biomolec. Spectr., 71, 1199 (2008)

[33] Zhang YZ, Xiang X, Mei P, Dai J, Zhang LL, Liu Y, Spectroc. Acta Pt. A-Molec. Biomolec. Spectr., 72, 907 (2009)

[34] Dufour C, Dangles O, Flavonoid: BBA Gen. Subj., 1721, 164 (2005)

[35] Mehra KS, Singh R, Bhatia RP, Sen PC, Singh H, Ann. Ophthalmol., 7, 1470 (1975)

[36] Daniel VP, Murukan B, Kumari BS, Mohanan K, Spectroc. Acta Pt. A-Molec. Biomolec. Spectr., 70, 403 (2008)

[37] Yalmaz M, Bayramo G, Ara CMY, Food Chem., 89, 11 (2005)

[38] Malle M, Denicola A, Biochem. Mol. Biol. Educ., 30, 309 (2002)