An electrode characteristic of a microelectrode array was fabricated by irradiating with UV light, through a photomask, a self-assembled monolayer of hexadecylthiolate formed on an Au electrode in air. Further, it was discovered that irradiation of the monolayer electrode in a K4Fe(CN)(6) solution allowed removal of the monolayer depending on the irradiation time. An electrochemical response characteristic of a microelectrode array appeared before complete removal of the monolayer took place. A microelectrode array was also fabricated by irradiating the monolayer electrode at selected places in a K4Fe(CN)(6) solution with a focused laser beam of the second harmonic of an Nd:YAG laser (532 nm). Cyclic voltammograms of the photo-fabricated electrodes are compared with those predicted by voltammetric theories.