Amperometric sensors for linamarin, a cyanogenic glycoside, were fabricated. They were based on the detection of glucose liberated from the hydrolysis of linamarin by linamarase from cassava leaf. A linamarase-glucose oxidase bienzyme-modified SnO2 electrode was observed to function at +900 mV vs. AgAgCl in a hydrogen peroxide detection mode, and could determine linamarin down to 10 mu M. In contrast, a linamarase-glucose oxidase bienzyme-modified graphite electrode mediated by poly(N-isopropylacrylamide-co-vinylferrocene) responded to greater than 0.2 mM linamarin at +500 mV vs. AgAgCl. A linamarase-glucose oxidase-peroxidase trienzyme electrode was also fabricated by coating a peroxidase-incorporated polypyrrole-modified SnO2 electrode with a linamarase-glucose oxidase cross-linked film, This trienzyme electrode, which is the most practical linamarin sensor, was able to function at a mild potential of +150 mV vs, AgAgCl, and has a sensitivity of 5 mu M linamarin.