To find out whether the 26 shade tabs of the Toothguide 3D Master can be grouped according to the five groups of lightness proposed by the manufacturer (Vita-Zahnfabrik) the three color coordinates L*, C*, and h were studied. Upper central incisor color was measured through the Easyshade Compact spectrophotometer using 1361 participants aged between 16 and 89. The color of the middle third was registered in the Toothguide 3D Master nomenclature and in the CIELCh-system. Principal Component Analysis and Cluster analysis, were applied for data processing. The statistical analysis reveals the existence of five cluster groups. Cluster 1 comprises samples 1M1 - 2L1.5 - 2M1 - 2R1.5 - 3L1.5 - 3M1. Cluster 2 comprises 1M2 - 2L2.5 - 2M2 - 2M3 - 2R2.5 -3M2 - 3L2.5. Cluster 3 comprises 3R1.5 - 4L1.5 - 4L2.5 - 4M1 - 4M2 - 4R1.5 - 5M1. Cluster 4 comprises 3M3 - 3R2.5 - 4M3 - 4R2.5. And, finally, Cluster 5 comprises: 5M2 - 5M3. The 26 shade tabs in the Toothguide 3D Master can be grouped into five clusters following coordinates L*, C*, and h resulting from the dental spectrophotometer Vita Easyshade compact. The shade tabs that compose each cluster do not belong to the same lightness group. (c) 2014 Wiley Periodicals, Inc. Col Res Appl, 40, 194-200, 2015