Ultrathin ZnO nanofilms based on Zn substrate were synthesized through a facile one-pot hydrothermal method. Then this prepared Zn-ZnO integrated electrode was directly used as work electrode to detect glucose and hydrazine in the solution, respectively. Exhilaratingly, the results show that the prepared Zn-ZnO integrated electrode has significant electrocatalytic activity toward the oxidation of glucose and reduction of hydrazine. As a result, Zn-ZnO integrated electrode exhibits a wide linear range from 1 uM to 19.2 mM for the detection of glucose with a low detection limit of 1 uM (S/N =3) and a wide linear range from 0.5 uM to 14.2 mM for the detection of hydrazine with a linear range from with a low detection limit of 0.5 uM (S/N = 3). What's more, the proposed sensor displays excellent selectivity, good stability, and satisfying repeatability. (C) 2014 Published by Elsevier Ltd.