LiNbO3 nanotubes are prepared by biotemplate-assisted synthetic route. The mesoporous LiNbO3 nanotubes can be an ideal platform to immobilize glucose oxidase (GOx) for glucose biosensing. As the GOx-biocatalyzed oxidation of glucose yield H2O2, and LiNbO3 can direct catalyze the generated H2O2. What's more, one dimensional channels will facilitate the diffusion of reactants and ensure adequate contact between glucose and enzymes. Thus the as-prepared biosensor exhibit high sensitivity (52.4 mu A.mM(-1).cm(-2)), fast response (<2 s) and excellent selectivity to glucose. The results suggest the potential implementation of LiNbO3 as a sensing platform for electrochemical detection of biology reagents. (C) 2014 Elsevier Ltd. All rights reserved.