In this paper, we will describe an original approach to develop an all-solid-state electrochemiluminescent (ECL) electrode for reagentless detection. It was constructed based on the immobilization of poly(luminol/aniline) nano-rods, which was synthesized in aqueous medium by a simple one-pot strategy. The properties of the copolymer were characterized by microscopic, electrochemical and spectrometric investigations. It was then immobilized onto the surface of indium tin oxide coated glass to act as a working electrode for ECL analysis with the flow-injection mode. The as-prepared ECL electrode exhibited strong and stable ECL background in alkaline aqueous solution and responded toward some oxidants or antioxidants such as H2O2 (to be found to enhance its ECL) or resveratrol (to be found to quench its ECL). On this basis, the built reagentless ECL flow-injection analytical system was successfully applied to evaluate the gross antioxidant activity of some fruits with satisfactory results. (C) 2014 Elsevier Ltd. All rights reserved.