A simple and sensitive method for domperidone (DP) determination has been developed by electropolymerizing a polymer film on the surface of glassy carbon electrode (GCE) in acidic solution using cyclic voltammetry. The modified sensor was characterized by Field Emission Scanning Electron Microscopy (FE-SEM) and Electrochemical Impedance Spectroscopy (EIS). The electrochemical measurements were carried out using square wave voltammetry and cyclic voltammetry. The modified sensor exhibited an excellent catalytic response towards the oxidation of DP with a well-defined oxidation peak at 840 mV. The modified sensor exhibited linear calibration curve for DP over a concentration range of 0.1 mu M to 100 mu M in phosphate buffer solution of pH 7.2 with detection limit of 12.0 nM. The sensor was capable to determine DP effectively without any interference from the common metabolites like ascorbic acid, uric acid, xanthine and hypoxanthine. The analytical utilities of the sensor have been demonstrated by determining the DP in human fluids and pharmaceutical samples. Further, the modified sensor displayed voltammetric responses with high sensitivity, good selectivity and reproducibility which make it suitable for clinical diagnosis. (C) 2014 Elsevier Ltd. All rights reserved.