In this study, a two dimensional CFD code has been developed to investigate entropy generation in turbulent mixed convection heat transfer flow of supercritical fluids. Since the fluid properties vary significantly under supercritical conditions, the changes of entropy generation are large. The contribution of each of the mechanisms of entropy production (heat transfer and energy dissipation) is compared in different regions of the flow. The results show that the mechanisms of entropy generation act differently in the near wall region within the viscous sub-layer and in the region away from the wall. The effects of the wall heat flux on the entropy generation are also investigated. (C) 2014 Elsevier Ltd. All rights reserved.