The objective of this study was to evaluate the adaptability of the UBD microorganism on the biodegradation of three kinds of different wastewater as one of the applications of bio-augmentation technique in lab-scale batch experiments by directly adding UBD into the bioreactor. After biodegradation, the removal of chemical oxygen demand was lower than 50%; on the other hand, the total removal of total petroleum hydrocarbons was maintained at 70% in coking wastewater with different initial chemical oxygen demand and total petroleum hydrocarbon. At the same time, the removal of chemical oxygen demand was up to 83.7 and 93.2% in crude oil-water emulsion and diesel-water emulsion, respectively, at the optimized biodegrade conditions. The chemical oxygen demand was degraded from 1,321.3 to 256.3 mg/L, meanwhile the total petroleum hydrocarbon was degraded from 65.69 to 4.98 mg/L in actual refinery sewage under the optimal biodegradation conditions that were 500 mu L/L (v/v) concentration of UBD and continuous aeration, which was much better than natural conditions.