Thermoacoustic engines are the devices that convert thermal energy into acoustic energy without moving parts. The main objective of this study is to analyze the performance of a thermoacoustic prime mover measured in terms of onset temperature difference, frequency, and pressure amplitude by varying resonator, stack length, and plate thickness. From the experiments, it is observed that onset temperature difference and pressure amplitude increases with increase in resonator and stack length with minimum plate thickness, whereas the frequency increases with decrease in resonator and stack length with higher plate thickness. The experimental results are compared with simulated results via Design Environment for Low Amplitude Thermoacoustic Energy Conversion software (Los Alamos National Laboratory, Los Alamos, New Mexico, USA).