While HCCI engines have many advantages such as better efficiency and lower emissions, the control of these engines is still complex. In this context, cycle-to-cycle control using oxidizing species such as ozone (O-3) presents a good potential. Only 1 ppm of O-3 has already a significant impact on the ignition. Predicting this effect as well as the low temperature combustion and ignition generally requires a detailed mechanism. Using CFD simulations with the Tabulation of Dynamic Adaptive Chemistry (TDAC) method and a mechanism including more than 1000 chemical species, we have predicted the effect of ozone seeding on the combustion of iso-octane in a HCCI engine. This paper presents the validation of the CFD simulations with a large range of ozone concentration and initial temperature. It shows that the TDAC method is able to reproduce accurately such complex chemical phenomena accounting for heat transfer and chemical reactions in a real geometry. (C) 2014 Elsevier Ltd. All rights reserved.