The screening for finding the lipid producer of indigenous Indonesian tropical microalgae that obtained from peat-land at Riau, Sumatra and seashore at Lombok has carried out. Preliminary screening was done by selection on faster growing algae from 44 (forty-four) isolates. 10 (ten) isolates were chosen for further analysis. Out of 10 selected isolates shows that five isolates have indication rapid growth and high content of lipid, furthermore those chose isolates were observed comprehensively, namely LIPI11-2-Al005, LIPI11-2-Al010, LIPI11-2-Al015, LIPI11-2-Al018, and LIPI11-2-Al019. The microalgae are belong to the eukaryotic microalgae and its seem to be closed to Chlorophytes that has cell nuclear with an envelope, light green chloroplast, thick cell wall, round and elongated cell features. Cultivation of the microalgae in laboratory condition (1 L) has shown that lipid content was about 20-35% base in cell dry weight. The highest lipid content was found in the isolate LIPI11-2-Al018 of 30.74% per dry weight cell. LIPI11-2-A1018 therefore was test for scale up cultivation reach the culture volume of 5 L in the cylindrical photobioreactor, and expected to be source of triglycerides and lipids for biodiesel ingredient. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.