A new amine-terminated nylon 6/66/1010 (AM-6/66/1010) copolyamide hot melt adhesive with the molecular weight of 21,000-96,000Da was synthesized and blocked with 1-hexadecylamine. The AM-6/66/1010 samples were characterized by H-1-NMR spectroscopy, differential scanning calorimetry (DSC), thermal gravimetric analysis, and gel permeation chromatography. The H-1-NMR spectroscopy demonstrated that 1-hexadecylamine functions as blocking agent in this polymerization. The DSC thermograms showed that the extra peak (peak (II)) and the melting peak (peak (I)) of AM-6/66/1010 are moving left and the glass transition temperature of AM-6/66/1010 decreases as the mole ratio alpha(H/C) (1-hexadecylamine/total carboxyl) increases. The thermal gravimetric analysis indicated that AM-6/66/1010 hot melt adhesive has a high thermal stability. The peel strength of the test specimen obtained by hot melt glutinous lining of AM-6/66/1010 or AC-6/66/1010 and fabric 6535 (65% PET, 35% cotton) was studied using T-peel tests, and the results showed that the washable and dry-cleaning resistant of AM-6/66/1010 copolyamide hot melt adhesive is better than that of carboxyl-terminated nylon 6/66/1010 (AC-6/66/1010) copolyamide hot melt adhesive.