AimsTo evaluate the impact of diet composition on colonization dynamics of Camp.jejuni and on related physiological parameters in the chicken intestine. Methods and ResultsA total of 54 1-day-old Ross 308 broiler chicks were randomly divided into three isocaloric and isonitrogenous dietary groups: maize-based (MB), wheat-based (WB) diet and wheat-based diet with NSP-degrading enzyme supplementation (WBES). Chickens were orally infected with 10(8)CFU Camp.jejuni on day 14, and samples (n=6) were collected on 7, 14 and 21days postinfection (DPI), respectively. Colony forming units of Camp.jejuni of caecum and jejunum, short-chain fatty acid (SCFA) concentrations, pH values of the caecum, jejunal histomorphology and viscosity of jejunal chymus were measured. In case of WBES diet, lower Camp.jejuni colonization 14 DPI, higher jejunal viscosity, higher total SCFA concentrations in the caecum and enhanced jejunal histomorphology were observed compared to those measured in chickens fed MB diet. ConclusionsThe WBES diet altered Camp.jejuni colonization dynamics in the chicken intestine which resulted by higher SCFA concentrations in the caecum and by the change of gut morphology. Significance and Impact of the StudyOur study proves that diet composition can modify Camp.jejuni colonization depending on sampling time point postinfection.