Bio-imaging of hydroxyl radicals in plant cells using the fluorescent molecular probe rhodamine B hydrazide, without any pretreatment

Asano M; Doi M; Baba K; Taniguchi M; Shibano M; Tanaka S; Sakaguchi M; Takaoka M; Hirata M; Yanagihara R; Nakahara R; Hayashi Y; Yamaguchi T; Matsumura H; Fujita Y

Journal of Bioscience and Bioengineering, Vol.118, No.1, 98-100, 2014

DOI10.1016/j.jbiosc.2013.12.015 Export Citation

Bio-imaging of hydroxyl radicals in plant cells using the fluorescent molecular probe rhodamine B hydrazide, without any pretreatment

Asano M, Doi M, Baba K, Taniguchi M, Shibano M, Tanaka S, Sakaguchi M, Takaoka M, Hirata M, Yanagihara R, Nakahara R, Hayashi Y, Yamaguchi T, Matsumura H, Fujita Y

Rhodamine B hydrazide can be used to detect hydroxyl radicals in plant cells. RBH was easily inserted into plant cells without any pretreatment, and specifically reacted with intracellular hydroxyl radicals produced by antimycin A. RBH will be a powerful tool for detecting hydroxyl radicals in plant cells. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Hydroxyl radical;Nicotiana tabacum BY-2;Onion epidermal cell;Rhodamine B hydrazide;Reactive oxygen species