Protease and fatty acids are known to serve as pathogenic factors against root-knot nematodes. Here, we utilized Karanja deoiled cake as a nitrogen source for the first time in induction of protease by Paecilomyces lilacinus. Fatty acids, especially butyric acid, have also been detected in the same fungal culture filtrate. (c) 2014, The Society for Biotechnology, japan. All rights reserved.