A recombinant fusion protein BVN-T beta 4 is successfully expressed in Escherichia coli, purified in the laboratory and applied onto the wounds of diabetic mice to investigate its effects on diabetic wound healing. Our results show that the recombinant protein BVN-T beta 4 can promote diabetic wound healing in the murine model. (c) 2014, The Society for Biotechnology, Japan. All rights reserved.