Nano-scale liquid chromatography coupled to tandem mass spectrometry using the multiple reaction monitoring mode based quantitative platform for analyzing multiple enzymes associated with central metabolic pathways of Saccharomyces cerevisiae using ultra fast mass spectrometry

Matsuda F; Ogura T; Tomita A; Hirano I; Shimizu H

Journal of Bioscience and Bioengineering, Vol.119, No.1, 117-120, 2015

DOI10.1016/j.jbiosc.2014.06.010 Export Citation

Nano-scale liquid chromatography coupled to tandem mass spectrometry using the multiple reaction monitoring mode based quantitative platform for analyzing multiple enzymes associated with central metabolic pathways of Saccharomyces cerevisiae using ultra fast mass spectrometry

Matsuda F, Ogura T, Tomita A, Hirano I, Shimizu H

A widely targeted quantitative proteome analysis of Saccharomyces cerevisiae enzymes was performed employing an ultra fast mass spectrometry (UFMS). Nano-liquid chromatography-UFMS analysis of trypsin digested peptide samples derived from yeast strains successfully determined relative abundances of 303 peptides of 137 proteins. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Quantitative proteomics;Ultra fast mass spectrometry;Central carbon metabolism;Saccharomyces cerevisiae;Enzyme expression