The solubilities of biotin in water, methanol, ethanol, acetic acid, dimethylformamide (DMF), and dimethyl sulfoxide (DMSO) were measured at temperatures ranging from 298.15 K to 333.85 K (p = 0.1 MPa) by the synthetic method with a laser monitoring dynamic technique. The mole fraction solubilities of biotin were observed to be highest in DMSO, and be lowest in water. The experimental data were well-correlated with the modified Apelblat equation, vant Hoff equation, and lambda h equation, in which the correlation coefficients (R-2) were noted in the range of 0.997 to 1.000 and the root-mean-square deviations (rmsd) were less than 2.82 %. Thermodynamic studies showed that dissolution of biotin was an endothermic and spontaneous process in all selected solvents.