Wood-based mesoporous activated carbon fibers (C-WACF) were prepared using wood charcoal (WC) as an additive. C-WACF with different WC contents (0, 0.5, 1, 1.5, 2 and 2.5 %) was characterized by N-2 adsorption, SEM, XRD, and XPS. With more WC added, the BET specific surface area and total volume of C-WACF showed upsurge and reached a maxima at WC = 1 %, when WC > 1 % a diminishing trend was seen. The mesoporosity of C-WACF reached 45 % when the WC content was 1 %. C-WACF reserved the characteristic peak of WC by XRD. With a WC content increase, the d (002) and L (a) of C-WACF were seen to decrease and then increase; L (c) and L (c)/d (002) first increased and then decreased. XPS showed more C-C and C-OOH groups transformed to C-OH and C=O groups on the surface of C-WACF with higher WC loading. The introduction of 1 % WC was proved to be appropriate for making Wood-based mesopores ACF.