Copolymers of N-alkylacrylamides and methacrylate bearing cholic acid pendant groups were synthesized via radical polymerization. The cholic acid pendant groups of such copolymers can form complexes with beta-cyclodextrin, and the effect of complexation on their thermoresponsive properties was studied. The phase transition temperatures (transition from hydrophilic to hydrophobic state) of the copolymers gradually increase with the addition of beta-cyclodextrin, due to the complexation of the cholic acid guest with the beta-cyclodextrin host. The increase of the phase transition temperature may be reversed by the addition of a competing guest molecule, potassium 1-adamantylcarboxylate. The host-guest complexation provides a straightforward way to vary the thermoresponsive properties of such copolymers.