In a perfusion-type support, axial dispersion caused by liquid mixing through the column, which might originate from some convective how through the throughpores of the support particles, contributed markedly to the spread of breakthrough curves. In comparison with conventional porous supports, in which the intraparticle mass transfer is rate limiting, the perfusion-type support was suitable for operations at high liquid flow rates with solutes of large molecular weight, because the spread of breakthrough curves was hardly affected by the liquid how rate and molecular weight of adsorbed components. Therefore, biomaterials of very low concentrations in feed solutions, such as bioactive materials secreted from animal cells, will be effectively purified by affinity chromatography by use of perfusion-type supports.