The combination of TiO2 photocatalyst with non-thermal gliding arc discharge for the degradation of organic pollutant Acid Orange 7 (AO7) was studied in aqueous solution. The effects of different parameters including initial concentration of TiO2, carrier gas source and initial pH value were studied. The variations of H2O2, O-3 and OH center dot active species and catalytic performance of reusing TiO2 were also investigated. It was shown that the optimum conditions for AO7 were TiO2 concentration of 1.0 g/L, oxygen as the carrier gas, and the initial pH value at acidic or basic. The crystal type of TiO2 didn't changed after repeated three times.