Prodn. of bio-EtOH of 1st and 2nd generation by conversion of wheat, beet molasses, maize and lignocellulosic biomass in 6 various factories was evaluated from environmental point of view as CO2 emission abatement during the whole life cycle (fertilization, transportation, fermentation). The highest decrease in CO2 emission was found for lignocellulosic biomass (lab. scale conditions) as well as for beet molasses and maize raw materials.