For consolidated bioprocessing (CBP), components of Jerusalem artichoke (Helianthus tuberosus L) tubers and stalks as a potential bioenergy crop were analyzed as carbon and nutrient sources, respectively. The effectiveness of chemical pretreatment with dilute acid or alkali was evaluated to develop a CBP method. Cellulose content, delignification, and enzymatic hydrolysis efficiency of the pretreated stalks were increased more effectively by NaOH treatment than dilute H2SO4 treatment. However, weight loss was greater during alkali pretreatment. Additionally, large volumes of water were required to wash the alkali-treated biomass. Therefore, CBP using the dilute acid-pretreated stalk and the ground tuber of Kluyveromyces marxianus were investigated. Fermentation of both pretreated stalks and tubers by K. marxianus with no nutrient supplementation proceeded acceptably. At 10% (w/v) stalk and 8% (w/v) tuber loading, K. marxianus produced 45.3 g/L ethanol after 30 h. The ethanol yield was 0.252 g ethanol per g dry biomass, or 0.32 g ethanol per g fermentable sugars, with a fermentable sugar conversion rate of 60%. These results suggest a cost-effective CBP strategy for bioethanol production from the whole Jerusalem artichoke plant. (C) 2013 Elsevier Ltd. All rights reserved.