The focus of this study is to investigate efficiency of the sulphate reducing passive bioreactors for the treatment of acid mine drainage. The performance of locally available substrates (manures, woodchips, millet fodders and sugarcane waste) were examined for the treatment of acid mine drainage. The manures showed efficient removal of metals and other pollutants. With cow manure, buffalo manure and goat manure pH increased from initial 2.70 to 6.25, 7.10 and 7.50. All cellulosic wastes also raised pH in a range of 4.83-5.32. Significant reduction was observed in Eh, acidity and sulphate concentration also. It was observed that: 51.49-99.32% of Fe, 84.95-99.97% of Cu, 35.11-99.78% of Zn, 17.87-99.14% of Ni, 63.55-99.02% of Co and 12.68-73.86% of Mn were removed in maximum retention period of 10 days. Thus manures can be cost effective as a single substrate in a passive treatment method for removal of metals from AMD in bioreactors. Also chemical makeup of substrate provided an insight on the ability of sulphate reduction and metal removal. (C) 2012 Elsevier Ltd. All rights reserved.