HAp, Zeolite에 여러 금속 Ion 치환시 나타나는 항균효과

김윤종; 김택남; 김상배; 조성백; 조건준; 이태형; Yun Jong Kim; Taik Nam Kim; Sang Bae Kim; Sung Baek Cho; Keon Joon Cho; Tae Hyung Lee

Korean Journal of Materials Research, Vol.11, No.2, 120-125, February, 2001

Export Citation

HAp, Zeolite에 여러 금속 Ion 치환시 나타나는 항균효과

Antimicrobial Effect of Metal tons Substitution to HAp, Zeolite

김윤종¹, 김택남^{2, 3}, 김상배¹, 조성백¹, 조건준¹, 이태형¹

¹한국지질자원연구소
²배재대학교 신소재공학부
³배재대학교 바이오 의약 연구센터

Yun Jong Kim¹, Taik Nam Kim^{2, 3}, Sang Bae Kim¹, Sung Baek Cho¹, Keon Joon Cho¹, and Tae Hyung Lee¹

¹Korea Institute of Geoscience & Mineral Resources, Taejeon 305-350, Korea
²Div. of Advanced Materials Engineering, Pai chai University, Taejon, 302-735, Korea
³Research Center for Biomedicinal Resources (Bio-Med RRC), Pai chai University, Taejon, 302-735, Korea

초록

흡착재료에는 hydroxyapatite(HAp), zeolite,, 분자체탄소, 활성탄, 알루미나등의 재료가 많이 사용되고 있다. 이들 흡착재료 중에서 금속이온과 양이온 교환반응이 좋은 HAp, zeolite는 폐수처리과정에서 많이 사용되고 있지만, 피러나 이 폐수처리과정에서 문제되는 것은 유해한 중금속뿐만 아니라 많은 세균들이 이 폐수에 존재하고 있어 수질오염 등의 여러 가지 문제를 야기 시키고 있다. 본 연구는 여러 중금속 흡착재료 중에서 HAp, zeolite에 항균효과가 있다고 알려진 금속이온 (Ag + , Cu 2+ , Zn 2+ )들을 이온 치환시켜 항균성을 흡착재료에 부여하고자 한다. HAp, zeolite에 Ag + , Cu 2+ , Zn 2+ 의 금속이온들을 치환시킨 후, E. Coli로 항균효과를 측정하였다. HAp, zeolite와 여러 금속이온 치환후의 항균효과를 측정한 결과, Ag + 로 이온치환 시킨 HAp, zeolite에서는 1 × 10 ?2 cell/ml 농도의 E. Coli에서 24시간가지 완벽한 항균효과를 보였고, Cu 2+ 와 Zn 2+ 으로 이온치환 시킨 경우에서는 좋은 항균효과를 보이지 않았다. 이러한 결과는 Feng. et. al.이 발표한 바와 같이1) Ag ion이 cell내부의 DNA에 영향을 기척, 복제능력을 떨어뜨리고 Cell을 비활성화 시키기 때문이다.

Generally, hydroxyapatite(HAp), zeolite, carbon molecular sieve , activated carbon and alumina are used as heavy metal ions adsorption materials. Among those adsorption materials, HAp which has good positive ion-exchange ability with metal ion, and zeolite are utilized in wastewater treatment. Most of water pollutions are caused by hazardous heavy metals ions as well as bacteria in waste water. In this study, a adsorption materials (HAP and zeolite) are ion-exchanged with a well known antimicrobial metal ions, such as Ag + ,Cu 2+ ,andZn 2+ , in order to give a adsorption of heavy metal ions and a killing effects of bacteria. The antimicrobial effects of adsorption materials are observed using by E. Coli. The results show that there is a complete antimicrobial effect in the adsorption materials with Ag + at the concentration of 1×10 ?4 cell/ mℓ of E. Coli until 24 hours. However, there is not good antimicrobial effects in the adsorption materials with Cu 2+ ,andZn 2+ substitution. Feng et. al. showed the denaturation effects of silver ions which induces the condensed DNA molecules and losing their replication abilities.

Keywords:HAp;zeolite;antimicrobial effect;ion-exchange

[References]

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[1] Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO, J. Biomed. Mater. Res., 52, 662 (2000)

[2] Monma H, 'Bioceramics-Apatite Bio-ceram-ics', Proceedings of the First Korea-Japan Semi-nar on New Ceramics, 176 (1984) (1984)

[3] Kim TN, Feng QL, Kim JO, Wu J, Wang H, Chen GQ, Cui FZ, J. Mat. Sci. Mat. Med., Antimicrobial Effects of Metal Ions (Ag + ,;Cu 2+ ,;Zn 2+ ) in Hydroxyapatite, 9, 129 (1998)

[4] Kim TN, Feng QL, Wu J, Kim JO, J. Korean Vaccum Society, Silver-impregnated HAp-coating on alumina substrate for prevention of infection, 6, 57 (1997)

[5] Breck DW, J. Chem. Educ, 41, 478 (1964)

[6] Smolka HG, Schwuger MJ, Colloid. Polymer Sci., 256, 270 (1978)

[7] Angell CL, J. Phys. Chem., 69, 3463 (1965)

[8] Sherry HS, J. Phys. Chem., The Ion-Exchange Properties of Zeo-lites, 70, 1158 (1966)

[9] Ikeda T, Sasakl M, Yasunaga T, J. Phys. Chem., Kinetic Studies of Ion Exchange of A Ikylammonium Ion for Sodium Ion in Aqueous Suspensions of Zeolite 4A Using the pressure-Jump Method, 87, 745 (1983)

[10] Gaus H, Lutze W, J. Phys. Chem., Kinetics of Sr/Ba and Sr/Ca Ion Exchange in Synthetic Zeolite A, 85, 79 (1981)

[11] Drummond D, Jonge AD, Rees LC, J. Phys. Chem., Ion-Ex-change Kinetics in Zeolite A, 87, 1967 (1983)

[12] Aoki H, 'Science and Medical Applications of Hydroxyapatite', JAAS. (1991) (1991)

[13] Kubaschewski O, Alcock CB, Metallurgical ther-mochemistry 5th Edition, pp. 282, 322, Int., Series on Mat., Sci., and Technology