화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.39, No.3, 493-498, May, 2015
DOI10.7317/pk.2015.39.3.493  Export Citation
오리발 유래 콜라겐 스펀지의 피부재생 효과: In vitro 연구
Effect of Duck’s Feet Derived Collagen Sponge on Skin Regeneration: In Vitro Study
차세롬, 정현기, 김수영, 김은영, 송정은, 박찬흠1, 권순용2, 강길선†
  • 전북대학교 BIN융합공학과, 고분자나노공학과, 고분자융합소재연구소
  • 1한림대학교 의과대학 춘천성심병원 이비인후과
  • 2가톨릭대학교 의과대학 여의도성모병원 정형외과
Se-Rom Cha, Hyun-Ki Jeong, Su-Young Kim, Eun-Young Kim, Jeong-Eun Song, Chan-Hum Park1, Soon-Yong Kwon2, and Gilson Khang†
  • Dept. of BIN Fusion Tech. & Dept. of polymerNano Sci. Tech., Chonbuk National University, 567 Baekje-daero, Jeonju 561-756, Korea
  • 1Dept. of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, 1-1 Okcheon, Chuncheon, Gangwon 200-702, Korea
  • 2Dept. of Orthepedic Surgery, Yeouido St. Mary’s Hospital, Catholic University of Korea, 62 Yeouido-dong Yeongdeungpo-gu, Seoul 150-896, Korea
E-mail:
초록
피부재생을 위한 생체재료는 염증반응이 최소화되는 안정한 소재로 빠른 피부재생을 돕기 위해 우수한 생체활성과 생체친화성을 가져야 하며, 세포의 부착과 성장을 돕는 미세구조와 다공성이 있어야 한다. 본 연구에서는 새로운 콜라겐 원으로서의 축산부산물인 오리발을 사용하여 콜라겐을 추출하였고 이를 탈미네랄화된 골분(demineralized bone powder, DBP), 돼지 소장점막하 조직(small intestinal submucosa, SIS)과 비교하기 위해 스펀지 형태로 제작하였다. 지지체의 물리, 화학적 특징은 SEM, FTIR을 통해 확인하였다. 세포를 파종하여 MTT를 통해 세포의 부착 및 증식률을 측정하였고, 전염증성 사이토카인의 발현도를 보기 위해 RT-PCR을 실시하였다. 또한 항산화 활성능력을 보기 위해 1,1-diphenyl-2-picrylhydrazyl(DPPH)를 측정하였다. 그 결과 오리발 콜라겐 지지체가 물리적 특성이 우수하고 생체적합성이며, 상처 치유제로서의 가능성을 보여주었다.
For biomaterials for skin regeneration with minimized inflammatory response, high bioactivity and biocompatibility are highly required. Also, it should have a porous microstructure to improve cell adhesion and growth. In this study, we extracted a new collagen source from duck's feet which is by-product, and made the shape of sponges from duck’s feet collagen (DC) to compare with DBP and SIS. To analyze physical and chemical property of the scaffold, SEM and FTIR were used. MTT assay was used to measure the attachment and proliferation of NIH/3T3 in the scaffolds. RTPCR was used to evaluate the expression of proinflammatory cytokine. Also, 1,1-diphenyl-2-picrylhydrazyl (DPPH) was used to measure the ability of antioxidant activity. Overall, this study shows that DC scaffold is biocompatible and has good physical property. Additionally, DC scaffold shows the potential as wound healing biomaterials.
Keywords:duck’s feet collagen (DC);skin regeneration;inflammatory
  1. Friess W, Eur. J. Pharm. Biopharm., 45, 113 (1998)
  2. Ng KW, Inter. J. Tissue Regen., 4, 29 (2013)
  3. Hollister SJ, Nat. Mater., 4(7), 518 (2005)
  4. Egana AL, Scheibel T, Biotechnol. Appl. Biochem., 55, 155 (2010)
  5. Metcalfe AD, Ferguson MW, J. R. Soc. Interface, 4, 413 (2007)
  6. Lee OJ, Lee JM, Jin HJ, Park CH, Inter. J. Tissue Regen., 1, 68 (2010)
  7. Ye Q, Ohsaki K, Li K, Li DJ, Zhu CS, Ogawa T, Tenshin S, Yamanoto TT, Auris Nasus Larynx, 28, 131 (2001)
  8. Liu C, Xia Z, Czernuszka JT, Chem. Eng. Res. Des., 85(A7), 1051 (2007)
  9. Singer AJ, Clark RA, N. Engl. J. Med., 341, 738 (1999)
  10. Caliari SR, Ramirez MA, Harely BAC, Biomaterials, 32, 8990 (2011)
  11. Aszodi A, Legate KR, Nakchbandi I, Fassler R, Annu. Rev. Biol., 55, 591 (2006)
  12. Song JE, Kim R, Lee JH, Kim HM, Kim CM, Lee D, Khang G, Inter. J. Tissue Regen., 4, 17 (2013)
  13. Brinckmann J, Top. Curr. Chem., 247, 1 (2005)
  14. Boehler RM, Shin S, Fast AG, Gower RM, Shea LD, Biomaterials, 34, 5431 (2013)
  15. Grotendorst GR, Seppa HE, Kleinman HK, Martin GR, Proc. Natl. Acad. Sci., 78, 3669 (1981)
  16. Ramshaw JA, Werkmeister JA, Glattauer V, Biotechnol. Genet. Eng. Rev., 13, 335 (1996)
  17. Chevallay B, Herbage D, Med. Biol. Eng. Comput., 38, 211 (2000)
  18. Powell HM, Supp DM, Boyce ST, Biomaterials, 29, 834 (2008)
  19. Capito RM, Spector M, Gene Ther., 14, 721 (2007)
  20. Murray JC, Stingl G, Kleinman HK, Martin GR, Katz SI, J. Cell Biol., 80, 197 (1979)
  21. Chong EJ, Phan TT, Lim IJ, Zhang YZ, Bay BH, Ramakrishna S, Lim CT, Acta Biomater., 3, 321 (2007)
  22. Oliver RF, Barker H, Cooke A, Grant RA, Biomaterials, 3, 38 (1982)
  23. Lynch SE, Colvin RB, Antoniades HN, J. Clin. Oncol., 84, 640 (1989)
  24. Kropp BP, Eppley BL, Prevel CD, Rippy MK, Harruff RC, Badylak SF, Adams MC, Rink RC, Keating MA, Urology, 46, 396 (1995)
  25. Mizuno S, Glowacki J, Biomaterials, 17, 1819 (1996)
  26. Boucard N, Viton C, Agay D, Mari E, Roger T, Chancerelle Y, Domard A, Biomaterials, 28, 3478 (2007)
  27. Yeo JH, Lee KG, Kim HC, Oh HYL, Kim AJ, Kim SY, Biol. Pharm. Bull., 23, 1220 (2000)
  28. Roh DH, Kang SY, Kim JY, Kwon YB, Kweon HY, Lee KG, Park YH, Baek RM, Heo CY, Choe J, J. Mater. Sci. -Mater. Med., 17, 547 (2006)
  29. Ko HA, Kim HM, Ha HJ, Kim R, Jang JE, Kim KH, Lee JH, Song JS, Yoon H, Lee OJ, Lee D, Park CH, Khang G, Inter. J. Tissue Regen., 9, 103 (2012)
  30. Kropp BP, Eppley BL, Prevel CD, Rippy MK, Harruff RC, Badylak SF, Keating MA, Urology, 43, 396 (1995)
  31. Park KS, Jin CM, Yun SJ, Hong KD, Kim SH, Kim MS, Rhee JM, Khang G, Lee HB, Polym.(Korea), 29(5), 501 (2005)
  32. Kim SH, Song JE, Lee D, Khang G, J. Tissue Eng. Regen. Med., 8, 279 (2014)
  33. Shim CR, Lee YJ, Ko HA, Kim MJ, Bae JW, Lee JH, Song JE, Khang G, Inter. J. Tissue Regen., 3, 50 (2012)
  34. Fernandez VG, Lavergne G, Berthoumieu P, Atom. Sprays, 21(1), 1 (2011)
  35. Molyneux P, Songklanakarin, J. Sci. Technol., 26, 211 (2004)