화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Applied Chemistry for Engineering, Vol.26, No.3, 265-269, June, 2015
DOI10.14478/ace.2015.1007  Export Citation
EMS (Ethyl Methane Sulphonate) 처리에 의한 Chlorella Vulgaris 변이주 생성 및 특성 분석
Characterization of Chlorella Vulgaris Mutants Generated byEMS (Ethyl Methane Sulphonate)
김옥주, 이재화1, †
  • (주) 클로랜드
  • 1신라대학교 의생명과학대학 제약공학
OK JU Kim, and Jae-Hwa Lee1, †
  • Chloland Co. Ltd., Geoje 656-851, Korea
  • 1Department of Pharmaceutical Engineering, College of Medical & Life Science, Silla University, Busan 617-736, Korea
E-mail:
초록
Chlorella vulgaris는 녹조류로 직경 2~10 μm의 구형 단세포식물로 단백질, 클로로필, 카로티노이드, 클로렐라 성장촉진인자(CGF)와 같은 기능성물질을 함유하고 있는 것으로 밝혀짐에 따라 영양학적 우수성이 확인되었으며, 간 기능개선 및 면역 기능강화와 같은 기능성이 보고되고 있다. 본 연구에서, C. vulgaris에 화학적 돌연변이원인 EMS (ethylmethane sulphonate) 처리에 따른 세포 성장, 색소 및 지질 함량에 미치는 영향을 살펴보았고, 그에 따른 변이주를 확보하였다. C. vulgaris에 EMS를 처리하여 얻어진 변이주를 E14, E24로 명명하였다. 세포 성장의 경우 mutant의 성장 패턴이 Wild Type (WT) 균주의 성장 패턴과 비슷하였다. E14, E24의 클로로필 함량은 각각 99, 52% 증가하였고, 카로티노 이드 함량은 E14는 7% 증가, E24의 경우 5% 감소하였다. 지질 함량의 경우 WT에 비해 E24의 경우 23% 증가하였고, E14는 12% 감소하였다. 이 결과, EMS에 의해 유도된 C. vulgaris 돌연변이주는 세포 성장은 차이를 보이지 않지만 클로로필과 카로티노이드, 지질 함량을 많이 축적하여 고부가가치 소재로 이용될 것으로 기대된다.
Chlorella vulgaris (C. vulgaris) is a spherical unicellular green algae and the diameter ranges from 2 to 10 μm. C. vulgaris possess nutritional excellence because it contains various functional materials including high protein contents, chlorophyll, carotenoid, and chlorella growth factor (CGF). In order to study effects of mutagen, ethyl methane sulphonate (EMS) was used as a chemical mutagen and some mutants could be obtained. We named 2 type mutants as E14 and E24 obtained after treating with EMS. In the cell growth, growth patterns of mutants were similar to those of the wild type. Chlorophyll contents of E14 and E24 increased up to 99 and 52%, respectively compared to those of the wild type. The carotenoid content of E14 increased to 7%, but the value of E24 decreased 5% compared to that of the wild type. For the lipid contents E24 increased to 23%, while E14 decreased 12% when compared to those of the wild type. As a result, there is no difference between the mutants and wild type in the cell growth, but considering that mutants contains more physiological materials than those of the wild type, we can expect the mutants of C. vulgaris could be used as important high added-value materials.
Keywords:Microalgae;Chlorella vulgaris;Mutants;EMS (Ethyl Methane Sulphonate)
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