Preparation of calcium chloride-loaded solid lipid particles and heat-triggered calcium ion release

Huangying Guo; Jin-Chul Kim

Korean Journal of Chemical Engineering, Vol.32, No.8, 1618-1624, August, 2015

DOI10.1007/s11814-015-0006-1 Export Citation

Preparation of calcium chloride-loaded solid lipid particles and heat-triggered calcium ion release

Huangying Guo, and Jin-Chul Kim^†

College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University, 192-1, Hyoja 2-dong, Chunchon, Kangwon-do 200-701, Korea

E-mail:

CaCl2-loaded solid lipid particles (SLPs) were prepared by a melt/emulsification/solidification method. CaCl2 microparticles (1-5 μm) could be obtained in a mortar with aid of the dispersant (Tween 80/Span80 (35/65, w/w)) when the ratio of CaCl2 to dispersant was 2 : 0.1 (w/w). SLP was prepared by dispersing 0.42 g of micronized CaCl2 particles in 2 g of molten PBSA, emulsifying the mixture at 85 oC in 40 ml of Tween 20 solution (0.5% w/v), and quenching the emulsion in an ice bath. The diameter of CaCl2-loaded SLP was 10-150 μm. The unenveloped CaCl2 could be removed by dialysis and the specific loading of CaCl2 in SLP was 0.036mg/mg. An EDS spectrum of CaCl2-loaded SLP, which was dialyzed, showed that the unenveloped CaCl2 was completely removed. Any excipients (dispersant, Tween 20, CaCl2) had little effect on the melting point of SLPs. No appreciable amount of Ca2+ was released in 20-50 oC for 22 h. But the release degree at 60 oC was significant (about 2.3%) during the same period. The matrix of the lipid particle was in a liquid state at 60 oC, so CaCl2 particles could move freely and contact the surrounding water, leading to the release. At 70 oC, the release degree at a given time was a few times higher than that obtained at 60 oC.

Keywords:Solid Lipid Particles;Calcium Chloride;Heat-triggered Release;Calcium Ion

[References]

Madene A, Jacquot M, Scher J, Desobry S, Int. J. Food Sci. Technol., 41, 1 (2006)
Aebischer P, Schluep M, Deglon N, Joseph JM, Hirt L, Heyd B, Goddard M, Hammang JP, Zurn AD, Kato AC, Regli F, Baetge EE, Nat. Med., 2, 696 (1996)
Freitas C, Muller RH, In. J. Pharmaceut., 168, 221 (1998)
Zimmermann E, Muller RH, Eur. J. Pharm. Biopharm., 52, 203 (2001)
Paasonen L, Romberg B, Storm G, Yliperttula M, Urtti A, Hennink WE, Bioconjugate Chem., 18, 2131 (2007)
Matsuoka S, Matsumori N, Murata M, Org. Biomol. Chem., 1, 3882 (2003)
Carbonea C, Tomaselloa B, Ruozib B, Renis M, Puglisi G, Eur. J. Med. Chem., 49, 110 (2012)
Mei Z, Li X, Wu Q, Hu S, Yang X, Pharmacol. Res., 51, 345 (2005)
Souto EB, Doktorovova S, Mira EG, Egea MA, Garcia ML, Curr. Eye Res., 35, 537 (2010)
Rudolph C, Schillinger U, Ortiz A, Tabatt K, Plank C, Muller RH, Rosenecker J, Pharm. Res-Dordr., 21, 1661 (2004)
Bondi’ ML, Azzolina A, Craparo EF, Lampiasi N, Capuano G, Giammona G, Cervello M, J. Drug Target., 15, 295 (2007)
Malmsten M, Soft Matter., 2, 760 (2006)
Jaina A, Agarwala A, Majumder S, Lariya N, Khaya A, Agrawal H, Majumdar S, Agrawal GP, J. Control. Release, 148, 359 (2010)
Atta AM, Abdel-Rauf ME, Maysour NE, Rahiem AMA, Abdel-Azim AA, J. Polym. Res., 13, 39 (2006)
Pouton CW, Porter CJH, Adv. Drug Deliv. Rev., 60, 625 (2008)
Gacesa P, Carbohydr. Polym., 8, 161 (1988)
Grant GT, Morris ER, Res DA, Smith JC, Thorm D, Febs Lett., 32, 195 (1973)

[Related Articles]

[1] Madene A, Jacquot M, Scher J, Desobry S, Int. J. Food Sci. Technol., 41, 1 (2006)

[2] Aebischer P, Schluep M, Deglon N, Joseph JM, Hirt L, Heyd B, Goddard M, Hammang JP, Zurn AD, Kato AC, Regli F, Baetge EE, Nat. Med., 2, 696 (1996)

[3] Freitas C, Muller RH, In. J. Pharmaceut., 168, 221 (1998)

[4] Zimmermann E, Muller RH, Eur. J. Pharm. Biopharm., 52, 203 (2001)

[5] Paasonen L, Romberg B, Storm G, Yliperttula M, Urtti A, Hennink WE, Bioconjugate Chem., 18, 2131 (2007)

[6] Matsuoka S, Matsumori N, Murata M, Org. Biomol. Chem., 1, 3882 (2003)

[7] Carbonea C, Tomaselloa B, Ruozib B, Renis M, Puglisi G, Eur. J. Med. Chem., 49, 110 (2012)

[8] Mei Z, Li X, Wu Q, Hu S, Yang X, Pharmacol. Res., 51, 345 (2005)

[9] Souto EB, Doktorovova S, Mira EG, Egea MA, Garcia ML, Curr. Eye Res., 35, 537 (2010)

[10] Rudolph C, Schillinger U, Ortiz A, Tabatt K, Plank C, Muller RH, Rosenecker J, Pharm. Res-Dordr., 21, 1661 (2004)

[11] Bondi’ ML, Azzolina A, Craparo EF, Lampiasi N, Capuano G, Giammona G, Cervello M, J. Drug Target., 15, 295 (2007)

[12] Malmsten M, Soft Matter., 2, 760 (2006)

[13] Jaina A, Agarwala A, Majumder S, Lariya N, Khaya A, Agrawal H, Majumdar S, Agrawal GP, J. Control. Release, 148, 359 (2010)

[14] Atta AM, Abdel-Rauf ME, Maysour NE, Rahiem AMA, Abdel-Azim AA, J. Polym. Res., 13, 39 (2006)

[15] Pouton CW, Porter CJH, Adv. Drug Deliv. Rev., 60, 625 (2008)

[16] Gacesa P, Carbohydr. Polym., 8, 161 (1988)

[17] Grant GT, Morris ER, Res DA, Smith JC, Thorm D, Febs Lett., 32, 195 (1973)