화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.23, No.8, 734-740, August, 2015
DOI10.1007/s13233-015-3099-9  Export Citation
Enhanced biological behavior of bacterial cellulose scaffold by creation of macropores and surface immobilization of collagen
Guangyao Xiong1, Honglin Luo1, 2, Chen Zhang3, Yong Zhu4, and Yizao Wan1, 2, †
  • 1School of Mechanical and Electrical Engineering, East China Jiaotong University, Nanchang, 330013, P. R. China
  • 2School of Materials Science and Engineering, Tianjin University, Tianjin, 300072, P. R. China
  • 3School of Optometry and Ophthalmology, Tianjin Medical University Eye Hospital, Tianjin, 300384, P. R. China
  • 4School of Chemical Engineering, Tianjin University, Tianjin, 300072, P. R. China
E-mail:
Bacterial cellulose (BC) is considered a promising three-dimensional (3D) nanofibrous scaffold for tissue engineering. To further improve its biological behavior, BC scaffold was modified by the creation of macropores and the immobilization of collagen (COL) on the surface. The creation of macropores was performed by laser perforation technique and the immobilization of collagen was achieved by solution immersion and subsequent crosslinking. The asprepared macroporous BC/COL nanocomposite (denoted as mBC/COL) was characterized by SEM, FTIR, contact angle measurement, and dynamic mechanical analysis, and its cell behavior was evaluated by MTT assay. SEM and FTIR confirmed the presence of collagen coating and patterned macropores (300 μm). Although the presence of macropores and collagen reduced its storage modulus and hydrophilicity, mBC/COL exhibited sufficient stiffness and wettability. More importantly, preliminary cell studies demonstrated that mBC/COL exhibited improved biological activity over BC and BC/COL due to the co-existence of macropores and collagen.
Keywords:bacterial cellulose;collagen;macropore;cell behavior;tissue engineering scaffold
  1. Tanpichai S, Quero F, Nogi M, Yano H, Young RJ, Lindstrom T, Sampson WW, Eichhorn SJ, Biomacromolecules, 13(5), 1340 (2012)
  2. Pertile RAN, Moreira S, Gil da Costa RM, Correia A, Guardao L, Gartner F, Vilanova M, Gama M, J. Biomater. Sci. Polym. Ed., 23, 1339 (2012)
  3. Saska S, Scarel-Caminaga RM, Teixeira LN, Franchi LP, Dos Santos RA, Gaspar AMM, de Oliveira PT, Rosa AL, Takahashi CS, Messaddeq Y, Ribeiro SJL, Marchetto R, J. Mater. Sci. -Mater. Med., 23, 2253 (2012)
  4. Backdahl H, Helenius G, Bodin A, Nannmark U, Johansson BR, Risberg B, Gatenholm P, Biomaterials, 27, 2141 (2006)
  5. Backdahl H, Esguerra M, Delbro D, Risberg B, Gatenholm P, J. Tissue Eng. Regen. Med., 2, 320 (2008)
  6. Zimmermann KA, LeBlanc JM, Sheets KT, Fox RW, Gatenholm P, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 31, 43 (2011)
  7. Czaja WK, Young DJ, Kawecki M, Brown RM, Biomacromolecules, 8(1), 1 (2007)
  8. Le Huec JC, Schaeverbeke T, Clement D, Faber J, Le Rebeller A, Biomaterials, 16, 113 (1995)
  9. Taboas JM, Maddox RD, Krebsbach PH, Hollister SJ, Biomaterials, 24, 181 (2003)
  10. Gao C, Wan Y, Yang C, Dai K, Tang T, Luo H, Wang J, J. Porous Mat., 18, 139 (2011)
  11. Gao C, Wan Y, Lei X, Qu J, Yan T, Dai K, Cellulose, 18, 1555 (2011)
  12. Kim HJ, Kim UJ, Leisk GG, Bayan C, Georgakoudi I, Kaplan DL, Macromol. Biosci., 7, 643 (2007)
  13. Xiong G, Luo H, Gu F, Zhang J, Hu D, Wan Y, J. Biomater. Nanobiotechnol., 4, 316 (2013)
  14. Zaborowska M, Bodin A, Backdahl H, Popp J, Goldstein A, Gatenholm P, Acta Biomater., 6, 2540 (2010)
  15. Martinez H, Brackmann C, Enejder A, Gatenholm P, J. Biomed. Mater. Res. Pt A, 100A, 948 (2012)
  16. Wang J, Yang C, Wan Y, Luo H, He F, Dai K, Huang Y, Soft Mater, 11, 173 (2013)
  17. Ahrem H, Pretzel D, Endres M, Conrad D, Courseau J, Mueller H, Jaeger R, Kaps C, Klemm DO, Kinne RW, Acta Biomater., 10, 1341 (2014)
  18. Luo HL, Xiong GY, Huang Y, He F, Wang W, Wan YZ, Mater. Chem. Phys., 110(2-3), 193 (2008)
  19. Saska S, Teixeira LN, de Oliveira PT, Gaspar AMM, Lima Ribeiro SJ, Messaddeq Y, Marchetto R, J. Mater. Chem., 22, 22102 (2012)
  20. Wang J, Wan YZ, Luo HL, Gao C, Huang Y, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 32, 536 (2012)
  21. Wang J, Wan Y, Han J, Lei X, Yan T, Gao C, Nano Lett., 6, 133 (2011)
  22. Chang ST, Chen LC, Lin SB, Chen HH, Food Hydrocoll., 27, 137 (2012)
  23. Lin WC, Lien CC, Yeh HJ, Yu CM, Hsu SH, Carbohydr. Polym., 94, 603 (2013)
  24. Dubey V, Pandey LK, Saxena C, J. Membr. Sci., 251(1-2), 131 (2005)
  25. Wang J, Wan Y, Huang Y, IET Nanobiotech, 6, 52 (2012)
  26. Wan Y, Gao C, Han M, Liang H, Ren K, Wang Y, Luo H, Polym. Adv. Technol., 22, 2643 (2011)
  27. Taokaew S, Seetabhawang S, Siripong P, Phisalaphong M, Materials, 6, 782 (2013)
  28. Phisalaphong M, Jatupaiboon N, Carbohydr. Polym., 74, 482 (2008)
  29. Jin J, Zuo G, Xiong G, Luo H, Li Q, Ma C, Li D, Gu F, Ma Y, Wan Y, J. Mater. Sci. Mater. Med., 25, 1025 (2014)
  30. Si HJ, Luo HL, Xiong GY, Yang ZW, Raman SR, Guo RS, Wan YZ, Macromol. Rapid Commun., 35(19), 1706 (2014)
  31. Ferreira AM, Gentile P, Chiono V, Ciardelli G, Acta Biomater., 8, 3191 (2012)
  32. Hong L, Wang YL, Jia SR, Huang Y, Gao C, Wan YZ, Mater. Lett., 60, 1710 (2006)
  33. Wan YZ, Hong L, Jia SR, Huang Y, Zhu Y, Wang YL, Jiang HJ, Compos. Sci. Technol., 66, 1825 (2006)
  34. Karageorgiou V, Kaplan D, Biomaterials, 26, 5474 (2005)
  35. Tsuruga E, Takita H, Itoh H, Wakisaka Y, Kuboki Y, J. Biochem., 121, 317 (1997)
  36. Gotz HE, Muller M, Emmel A, Holzwarth U, Erben RG, Stangl R, Biomaterials, 25, 4057 (2004)
  37. Grande CJ, Torres FG, Gomez CM, Troncoso OP, Canet-Ferrer J, Martinez-Pastor J, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 29, 1098 (2009)
  38. Schindler C, Williams BL, Patel HN, Thomas V, Dean DR, Polymer, 54(25), 6824 (2013)
  39. Pietrucha K, Int. J. Biol. Macromol., 36, 299 (2005)
  40. Jose MV, Thomas V, Johnson KT, Dean DR, Nyairo E, Acta Biomater., 5, 305 (2009)
  41. Pereira JDAS, Camargo RCT, Filho JCSC, Alves N, Rodriguez-Perez MA, Constantino CJL, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 36, 226 (2014)
  42. Cai ZJ, Yang G, J. Appl. Polym. Sci., 120(5), 2938 (2011)
  43. Martinez-Ramos C, Valles-Lluch A, Verdugo JMG, Ribelles JLG, Barcia JA, Orts AB, Lopez JMS, Pradas MM, J. Biomed. Mater. Res. A, 100A, 3276 (2012)
  44. Fukuda Y, Wang W, Ichinose S, Katakura H, Mukai T, Takakuda K, J. Biomed. Mater. Res. A, 102, 674 (2014)