Optimization of lipid extraction from marine green macro-algae as biofuel resources

Gwi-Taek Jeong; Don-Hee Park

Korean Journal of Chemical Engineering, Vol.32, No.12, 2463-2467, December, 2015

DOI10.1007/s11814-015-0083-1 Export Citation

Optimization of lipid extraction from marine green macro-algae as biofuel resources

Gwi-Taek Jeong^†, and Don-Hee Park¹

Department of Biotechnology, Pukyong National University, Busan 608-737, Korea
¹Department of Biotechnology and Bioengineering, Interdisciplinary Program of Graduate, School for Bioenergy and Biomaterials, Chonnam National University, Gwangju 500-757, Korea

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The extraction of lipid from Enteromorpha intestinalis was investigated. Among 13 types of organic solvent, the chloroform/methanol (2 : 1, v/v) system obtained the highest content. Considering the lipid extraction procedure and extracted amount, the optimal solid/liquid ratio was 1 : 50 (w/v). With increasing extraction temperature, the content was enhanced until 45 oC, after which it increased only slightly. Correspondingly, with increasing extraction time, the content linearly increased until 2.5 hr, after which it held steady. From a fatty acid compositional analysis of the lipid extracted from E. intestinalis, the content of palmitic acid and stearic acid was 39.85±1.19% and 14.61±0.80%, respectively. The unsaturated fatty acids (C18:1, C18:2 and C18:3) concentrations, meanwhile, were 0.3-3.2%.

Keywords:Enteromorpha intestinalis;Lipids;Bioenergy;Extraction;Operation Factor;Marine Macro-algae

[References]

Jang JS, Cho Y, Jeong GT, Kim SK, Bioprocess. Biosyst. Eng., 35, 11 (2012)
Meinita MDN, Hong YK, Jeong GT, Bioprocess. Biosyst. Eng., 35, 123 (2012)
Chandini SK, Ganesan P, Suresh PV, Bhaskar N, J. Food Sci. Technol., 45, 1 (2008)
Han YB, Edible Seaweed II - Components and biological activity, Korea University Press, Korea (2010).
Jeong GT, Park DH, Appl. Biochem. Biotechnol., 161(1-8), 41 (2010)
Jeong GT, Park DH, KSBB J., 46, 341 (2011)
Choi DB, Sim HS, Piao YL, Ying W, Cho H, J. Ind. Eng. Chem., 15(1), 12 (2009)
Song BB, Kim SK, Jeong GT, KSBB J., 26, 347 (2011)
Jeong GT, Yang HS, Park DH, Bioresour. Technol., 100(1), 25 (2009)
Gonzalez-Fernandez C, Sialve B, Bernet N, Steyer JP, Bioresour. Technol., 110, 610 (2012)
Prabakaran P, Ravindran AD, Lett. Appl. Microbiol., 53, 150 (2011)
Suganya T, Renganathan S, Bioresour. Technol., 107, 319 (2012)
Bligh EG, Dyer WJ, Can. J. Biochem. Physiol., 37, 911 (1959)
Kumari P, Reddy CR, Jha B, Anal. Biochem., 415, 134 (2011)
Martins AP, Yokoya NS, Colepicolo P, Braz. J. Phamacogn., 22(4), 854 (2012)
Xiao M, Shin HJ, Dong Q, Korean J. Chem. Eng., 30(12), 2119 (2013)
Suganya T, Gandhi NN, Renganathan S, Bioresour. Technol., 128, 392 (2013)
Miao XL, Wu QY, Bioresour. Technol., 97(6), 841 (2006)
Christie WW, in Advances in lipid methodology, Christie WW, Ed., Oily Press, Dundee (1993).
Ramluckan K, Moodley KG, Bux F, Fuel, 116, 103 (2014)
Kim DH, Jeong GT, KSBB J., 29(2), 92 (2014)
Denery JR, Dragull K, Tang CS, Li QX, Anal. Chim. Acta, 501, 175 (2004)

[Referenced By]

[1] Jang JS, Cho Y, Jeong GT, Kim SK, Bioprocess. Biosyst. Eng., 35, 11 (2012)

[2] Meinita MDN, Hong YK, Jeong GT, Bioprocess. Biosyst. Eng., 35, 123 (2012)

[3] Chandini SK, Ganesan P, Suresh PV, Bhaskar N, J. Food Sci. Technol., 45, 1 (2008)

[4] Han YB, Edible Seaweed II - Components and biological activity, Korea University Press, Korea (2010).

[5] Jeong GT, Park DH, Appl. Biochem. Biotechnol., 161(1-8), 41 (2010)

[6] Jeong GT, Park DH, KSBB J., 46, 341 (2011)

[7] Choi DB, Sim HS, Piao YL, Ying W, Cho H, J. Ind. Eng. Chem., 15(1), 12 (2009)

[8] Song BB, Kim SK, Jeong GT, KSBB J., 26, 347 (2011)

[9] Jeong GT, Yang HS, Park DH, Bioresour. Technol., 100(1), 25 (2009)

[10] Gonzalez-Fernandez C, Sialve B, Bernet N, Steyer JP, Bioresour. Technol., 110, 610 (2012)

[11] Prabakaran P, Ravindran AD, Lett. Appl. Microbiol., 53, 150 (2011)

[12] Suganya T, Renganathan S, Bioresour. Technol., 107, 319 (2012)

[13] Bligh EG, Dyer WJ, Can. J. Biochem. Physiol., 37, 911 (1959)

[14] Kumari P, Reddy CR, Jha B, Anal. Biochem., 415, 134 (2011)

[15] Martins AP, Yokoya NS, Colepicolo P, Braz. J. Phamacogn., 22(4), 854 (2012)

[16] Xiao M, Shin HJ, Dong Q, Korean J. Chem. Eng., 30(12), 2119 (2013)

[17] Suganya T, Gandhi NN, Renganathan S, Bioresour. Technol., 128, 392 (2013)

[18] Miao XL, Wu QY, Bioresour. Technol., 97(6), 841 (2006)

[19] Christie WW, in Advances in lipid methodology, Christie WW, Ed., Oily Press, Dundee (1993).

[20] Ramluckan K, Moodley KG, Bux F, Fuel, 116, 103 (2014)

[21] Kim DH, Jeong GT, KSBB J., 29(2), 92 (2014)

[22] Denery JR, Dragull K, Tang CS, Li QX, Anal. Chim. Acta, 501, 175 (2004)