화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.23, No.11, 994-1003, November, 2015
DOI10.1007/s13233-015-3131-0  Export Citation
Preparation of UV-curable gelatin derivatives for drug immobilization on polyurethane foam: Development of wound dressing foam
Hee-Dong Woo, Kyoung-Tae Park, Eun-Hye Kim, Yun Heo, Jin-Hong Jeong, Do-Gi Pyun1, Chang-Soon Choi2, Jae-Gwan Lee, Dong-Keun Han3, Jae-Woon Nah4, and Tae-Il Son†
  • Department of Systems Biotechnology and Bio-Environmental Technology (BET) Research Institute, Chung-Ang University, Anseong, Gyeonggi, 456-756, Korea
  • 1Department of Polymer Science and Engineering, Sungkyunkwan University, Suwon, Gyeonggi, 440-746, Korea
  • 2Department of Food and Nutrition, School of Food Science and Technology, Chung-Ang University, Anseong, Gyeonggi, 456-756, Korea
  • 3Center for Biomaterials, Korea Institute of Science and Technology, Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul, 136-791, Korea
  • 4Department of Polymer Science and Engineering, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam, 540-950, Korea
E-mail:
UV-curable azidophenyl-fish gelatin (azidophenyl-f.gel) for developing a wound-dressing foam was prepared by introducing the photo-reactive azidophenyl group of N-4-(azido)-benzoyloxysuccinimide (ABS) to fish gelatin. The structure of prepared azidophenyl-f.gel was identified by 1H NMR and FTIR. The biocompatibility of azidophenyl-f.gel was demonstrated through a cell cytotoxicity experiment. In order to demonstrate that azidophenyl-f.gel is photo-cured on polyurethane foam by UV irradiation, a patterning test was conducted, and the protein immobilization of azidophenyl-f.gel was demonstrated through fluorescence microscopy by using FITC-BSA. The protein release pattern in relation to the concentration of azidophenyl-f.gel and the number of underlying layers of polyurethane foam was observed, and the effect of the protein release pattern on cell growth was observed through cell proliferation. Water absorbency and moisture vapour transmission rate (MVTR) tests were conducted to measure the performance of the wound dressing foam, and a wound healing effect was observed through an in vivo animal experiment. These results demonstrate that UV-curable azidophenyl-f.gel is biocompatible and can immobilize protein by UV irradiation. It is a useful material that can be applied to the underlying layer of wound dressing foam to induce rapid wound healing by gradually releasing immobilized protein.
Keywords:azidophenyl-fish gelatin;wound dressing foam;epidermal growth factor (EGF);photo-cured;immobilization
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