Solar-to-chemical conversion platform by Robust Cytochrome P450-P(3HB) complex

Jae Hyung Lee; Dong Heon Nam; Sahng Ha Lee; Jong Hyun Park; Chan Beum Park; Ki Jun Jeong

Journal of Industrial and Engineering Chemistry, Vol.33, 28-32, January, 2016

DOI10.1016/j.jiec.2015.10.002 Export Citation

Solar-to-chemical conversion platform by Robust Cytochrome P450-P(3HB) complex

Jae Hyung Lee, Dong Heon Nam¹, Sahng Ha Lee¹, Jong Hyun Park, Chan Beum Park^{1, †}, and Ki Jun Jeong^†

Department of Chemical and Biomolecular Engineering (BK Plus program), Korea Advanced Institute of Science and Technology (KAIST), 291, Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea
¹Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291, Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea

E-mail:,

Cytochrome P450 monooxygenases which catalyze a remarkable variety of oxidative transformation are of exceptional interest for the synthesis of fine chemicals. However, due to the instability and requirement of expensive cofactor, P450s have yet to be extensively used for industry. Here, we developed a new platform that combines a solar-driven cofactor regeneration and immobilization of P450 on biopolymer. Through photochemical cofactor regeneration using eosin Y as a photosensitizer, P450-P(3HB) complex successfully catalyzed a O-dealkylation reaction. Furthermore, using the P450-P(3HB) complex and a solar-tracking module, we achieved a P450-catalyzed reaction under natural sunlight for four consecutive days in a preparative scale.

Keywords:Cytochrome P450 monooxygenases;Eosin Y;Poly(3-hydroxybutyrate);Solar-tracking system

[References]

Kille S, Zilly FE, Acevedo JP, Reetz MT, Nat. Chem., 3, 738 (2011)
Barry SM, Kers JA, Johnson EG, Song L, Aston PR, Patel B, Krasnoff SB, Crane BR, Gibson DM, Loria R, Challis GL, Nat. Chem. Biol., 8, 814 (2012)
Coelho PS, Brustad EM, Kannan A, Arnold FH, Science, 339(6117), 307 (2013)
Coelho PS, Wang ZJ, Ener ME, Baril SA, Kannan A, Arnold FH, Brustad EM, Nat. Chem. Biol., 9, 485 (2013)
Liu W, Wang P, Biotechnol. Adv., 25, 369 (2007)
Jeong KJ, Lee SY, Appl. Environ. Microbiol., 69, 1295 (2003)
Zhao H, van der Donk WA, Curr. Opin. Biotechnol., 14, 583 (2003)
Hollmann F, Hofstetter K, Schmid A, Trends Biotechnol., 24, 163 (2006)
De Wildema SM, Sonke T, Schoemaker HE, May O, Acc. Chem. Res., 40, 1260 (2007)
Shaked Z, Whitesides GM, J. Am. Chem. Soc., 102, 7104 (1980)
Johannes TW, Woodyer RD, Zhao HM, Biotechnol. Bioeng., 96(1), 18 (2007)
Hollmann F, Schmid A, Steckhan E, Angew. Chem.-Int. Edit., 40, 169 (2001)
Gust D, Moore TA, Moore AL, Acc. Chem. Res., 42, 1890 (2009)
Gust D, Moore TA, Moore AL, Acc. Chem. Res., 34, 40 (2001)
Kim JH, Nam DH, Park CB, Curr. Opin. Biotechnol., 28, 1 (2014)
Lee SH, Kim JH, Park CB, Chemistry, 19, 4392 (2013)
Yoshida T, Terada K, Schlettwein D, Oekermann T, Sugiura T, Minoura H, Adv. Mater., 12(16), 1214 (2000)
Li Q, Chen L, Lu G, J. Phys. Chem. C, 111, 11494 (2007)
Lee SH, Nam DH, Kim JH, Baeg JO, Park CB, Chembiochem, 10, 1621 (2009)
Xu X, Wei W, Huang M, Yao L, Liu S, Chem. Commun., 48, 7802 (2012)
Xu X, Qian J, Yu J, Zhang Y, Liu S, Chem. Commun., 50, 7607 (2014)
Lu J, Li H, Cui D, Zhang Y, Liu S, Anal. Chem., 86, 8003 (2014)
Cui DM, Mi L, Xu X, Lu JS, Qian J, Liu SQ, Langmuir, 30(39), 11833 (2014)
Lee JH, Nam DH, Lee SH, Park JH, Park SJ, Lee SH, Park CB, Jeong KJ, Bioconjugate Chem., 25, 2101 (2014)
Hollmann F, Witholt B, Schmid A, J. Mol. Catal. B-Enzym., 19-20, 167 (2002)
Lee JH, Lee SH, Yim SS, Kang KH, Lee SY, Park SJ, Jeong KJ, Appl. Biochem. Biotechnol., 170(7), 1767 (2013)
Abe R, Hara K, Sayama K, Domen K, Arakawa H, J. Photochem. Photobiol. A-Chem., 137, 63 (2000)
Kim SS, Yum JH, Sung YE, Sol. Energy Mater. Sol. Cells, 79(4), 495 (2003)
Lee SH, Kwon YC, Kim DM, Park CB, Biotechnol. Bioeng., 110(2), 383 (2013)
Lo HC, Leiva C, Buriez O, Kerr JB, Olmstead MM, Fish RH, Inorg. Chem., 40(26), 6705 (2001)
Poizat M, Arends IWCE, Hollmann F, J. Mol. Catal. B-Enzym., 63, 149 (2010)
Park JH, Lee SH, Cha GS, Choi DS, Nam DH, Lee JH, Lee JK, Yun CH, Jeong KJ, Park CB, Angew. Chem.-Int. Edit., 54, 969 (2015)
Chen D, Shen Y, Huang Z, Li B, Xie S, Int. J. Photoenergy, 2012, 6 (2012)
Velmurugan N, Sung M, Yim SS, Park MS, Yang JW, Jeong KJ, Bioresour. Technol., 138, 30 (2013)

[Referenced By]

[Related Articles]

[1] Kille S, Zilly FE, Acevedo JP, Reetz MT, Nat. Chem., 3, 738 (2011)

[2] Barry SM, Kers JA, Johnson EG, Song L, Aston PR, Patel B, Krasnoff SB, Crane BR, Gibson DM, Loria R, Challis GL, Nat. Chem. Biol., 8, 814 (2012)

[3] Coelho PS, Brustad EM, Kannan A, Arnold FH, Science, 339(6117), 307 (2013)

[4] Coelho PS, Wang ZJ, Ener ME, Baril SA, Kannan A, Arnold FH, Brustad EM, Nat. Chem. Biol., 9, 485 (2013)

[5] Liu W, Wang P, Biotechnol. Adv., 25, 369 (2007)

[6] Jeong KJ, Lee SY, Appl. Environ. Microbiol., 69, 1295 (2003)

[7] Zhao H, van der Donk WA, Curr. Opin. Biotechnol., 14, 583 (2003)

[8] Hollmann F, Hofstetter K, Schmid A, Trends Biotechnol., 24, 163 (2006)

[9] De Wildema SM, Sonke T, Schoemaker HE, May O, Acc. Chem. Res., 40, 1260 (2007)

[10] Shaked Z, Whitesides GM, J. Am. Chem. Soc., 102, 7104 (1980)

[11] Johannes TW, Woodyer RD, Zhao HM, Biotechnol. Bioeng., 96(1), 18 (2007)

[12] Hollmann F, Schmid A, Steckhan E, Angew. Chem.-Int. Edit., 40, 169 (2001)

[13] Gust D, Moore TA, Moore AL, Acc. Chem. Res., 42, 1890 (2009)

[14] Gust D, Moore TA, Moore AL, Acc. Chem. Res., 34, 40 (2001)

[15] Kim JH, Nam DH, Park CB, Curr. Opin. Biotechnol., 28, 1 (2014)

[16] Lee SH, Kim JH, Park CB, Chemistry, 19, 4392 (2013)

[17] Yoshida T, Terada K, Schlettwein D, Oekermann T, Sugiura T, Minoura H, Adv. Mater., 12(16), 1214 (2000)

[18] Li Q, Chen L, Lu G, J. Phys. Chem. C, 111, 11494 (2007)

[19] Lee SH, Nam DH, Kim JH, Baeg JO, Park CB, Chembiochem, 10, 1621 (2009)

[20] Xu X, Wei W, Huang M, Yao L, Liu S, Chem. Commun., 48, 7802 (2012)

[21] Xu X, Qian J, Yu J, Zhang Y, Liu S, Chem. Commun., 50, 7607 (2014)

[22] Lu J, Li H, Cui D, Zhang Y, Liu S, Anal. Chem., 86, 8003 (2014)

[23] Cui DM, Mi L, Xu X, Lu JS, Qian J, Liu SQ, Langmuir, 30(39), 11833 (2014)

[24] Lee JH, Nam DH, Lee SH, Park JH, Park SJ, Lee SH, Park CB, Jeong KJ, Bioconjugate Chem., 25, 2101 (2014)

[25] Hollmann F, Witholt B, Schmid A, J. Mol. Catal. B-Enzym., 19-20, 167 (2002)

[26] Lee JH, Lee SH, Yim SS, Kang KH, Lee SY, Park SJ, Jeong KJ, Appl. Biochem. Biotechnol., 170(7), 1767 (2013)

[27] Abe R, Hara K, Sayama K, Domen K, Arakawa H, J. Photochem. Photobiol. A-Chem., 137, 63 (2000)

[28] Kim SS, Yum JH, Sung YE, Sol. Energy Mater. Sol. Cells, 79(4), 495 (2003)

[29] Lee SH, Kwon YC, Kim DM, Park CB, Biotechnol. Bioeng., 110(2), 383 (2013)

[30] Lo HC, Leiva C, Buriez O, Kerr JB, Olmstead MM, Fish RH, Inorg. Chem., 40(26), 6705 (2001)

[31] Poizat M, Arends IWCE, Hollmann F, J. Mol. Catal. B-Enzym., 63, 149 (2010)

[32] Park JH, Lee SH, Cha GS, Choi DS, Nam DH, Lee JH, Lee JK, Yun CH, Jeong KJ, Park CB, Angew. Chem.-Int. Edit., 54, 969 (2015)

[33] Chen D, Shen Y, Huang Z, Li B, Xie S, Int. J. Photoenergy, 2012, 6 (2012)

[34] Velmurugan N, Sung M, Yim SS, Park MS, Yang JW, Jeong KJ, Bioresour. Technol., 138, 30 (2013)