In-vitro Anticancer and Antioxidant Activity of Gold Nanoparticles Conjugate with Tabernaemontana divaricata flower SMs Against MCF -7 Breast Cancer Cells

Preetam Raj J. P; Purushothaman M; Ameer Khusro; Shirly George Panicker

Korean Chemical Engineering Research, Vol.54, No.1, 75-80, February, 2016

DOI10.9713/kcer.2016.54.1.75 Export Citation

In-vitro Anticancer and Antioxidant Activity of Gold Nanoparticles Conjugate with Tabernaemontana divaricata flower SMs Against MCF -7 Breast Cancer Cells

Preetam Raj J. P^{1, †}, Purushothaman M¹, Ameer Khusro¹, and Shirly George Panicker^{1, 2}

¹Department of Plant Biology and Biotechnology, PG Biotechnology, Loyola College, Chennai-600 034.Tamil Nadu, India
²Helen Keller Research Centre, Loyola College, Chennai, Tamil Nadu, India

E-mail:

Biologically stabilized gold nanoparticles were synthesized from the flower aqueous extract of T. divaricata. The synthesized nanoparticles were characterized by UV-Vis spectrophotometer, Zeta sizer, FTIR and TEM analysis. T. divaricata reduced gold nanoparticles having particle size and potential of 106.532 nm and -10.2 mV, respectively, with a characteristic peak of 550 nm in UV-visible spectrophotometer. FTIR graph after comparison between the crude flower extract and gold nanoparticles showed three major shifts in the functional groups. The morphology and size of the gold nanoparticles were examined by HRTEM analysis, which showed that most of the nanoparticles were nearly spherical with size of 100 nm. The gold nanoparticles synthesized demonstrated potent anticancer activity against MCF-7 cell line. The findings conclude that the antioxidant molecule present in T. divaricata may be responsible for both reduction and capping of gold nanoparticles which possess potential applications in medicine and pharmaceutical fields.

Keywords:Anticancer;T. divaricata;Bioreduction;Breast cancer;Antioxidant;Gold nanoparticles

[References]

Ma X, Yu H, J. Bio. Med., 79(3), 85 (2006)
Tanih NF, Ndip RN, J. Scientific World, 7 (2013)
Amruthraj NJ, Preetam Raj JP, Lebel A, Appl. Nanoscience, 5, 403 (2015)
Yang HC, Hon MH, J. Microchem., 92, 87 (2009)
Huo Q, Colloids Surf. B: Biointerfaces, 59, 1 (2007)
Song JY, Kim B, Bioprocess. Biosyst. Eng., 32, 79 (2008)
Kumar V, Yadav SC, Yadav SK, J. Chem. Technol. Biotechnol., 85(10), 1301 (2010)
Singh B, Sharma RA, Vyas GK, Br., 125-132(2013).
Abel EE, John Poonga PR, Panicker S, Appl Nanosci (2015)
Mossman TJ, J. Immunol. Methods, 64, 55 (1983)
Sinha KA, Anal. Biochem., 47, 389 (1972)
Ebrahimzadeh MA, Nabavi SF, Nabavi SM, Pharmacol., 2, 1097 (2009)
Rio DD, Pellegrini N, Colombi B, Bianchi M, Serafini M, Torta F, Tegoni M, Musci M, Brighenti F, Clin Chem., 49, 690 (2003)
Abeer S, IMSA., 25(3) (2012)
Buzea C, Ivan I, Blandino P, Bobbie K, Biointerphases., 2(4), MR17-MR172(2007).
Basavegowda N, Kupiec A, Malina D, Yathirajan HS, Keerthi VR, Chandrasekar N, Dinkar S, Liny P, Adv. Mater. Lett., 4, 332 (2013)
Elavazhagan T, Arunachalam KD, Int. J. Nanomed., 6, 1265 (2011)
Rajesh WK, Jaya RL, Niranjan SK, Vijay DM, Sahebrao BK, Curr. Nanosci., 5, 117 (2009)
Ankamwar B, Damle C, Ahmed A, Sastry M, J. Nanosci. Nanotechnol., 5, 1665 (2005)
Sivakumar P, Nethradevi C, Renganathan S, Asian J. Pharm. Clin. Red., 5(4), 97 (2012)
Poornima K, Gopalakrishnan VK, Chin. J. Biology, 1(2), 1 (2012)

[1] Ma X, Yu H, J. Bio. Med., 79(3), 85 (2006)

[2] Tanih NF, Ndip RN, J. Scientific World, 7 (2013)

[3] Amruthraj NJ, Preetam Raj JP, Lebel A, Appl. Nanoscience, 5, 403 (2015)

[4] Yang HC, Hon MH, J. Microchem., 92, 87 (2009)

[5] Huo Q, Colloids Surf. B: Biointerfaces, 59, 1 (2007)

[6] Song JY, Kim B, Bioprocess. Biosyst. Eng., 32, 79 (2008)

[7] Kumar V, Yadav SC, Yadav SK, J. Chem. Technol. Biotechnol., 85(10), 1301 (2010)

[8] Singh B, Sharma RA, Vyas GK, Br., 125-132(2013).

[9] Abel EE, John Poonga PR, Panicker S, Appl Nanosci (2015)

[10] Mossman TJ, J. Immunol. Methods, 64, 55 (1983)

[11] Sinha KA, Anal. Biochem., 47, 389 (1972)

[12] Ebrahimzadeh MA, Nabavi SF, Nabavi SM, Pharmacol., 2, 1097 (2009)

[13] Rio DD, Pellegrini N, Colombi B, Bianchi M, Serafini M, Torta F, Tegoni M, Musci M, Brighenti F, Clin Chem., 49, 690 (2003)

[14] Abeer S, IMSA., 25(3) (2012)

[15] Buzea C, Ivan I, Blandino P, Bobbie K, Biointerphases., 2(4), MR17-MR172(2007).

[16] Basavegowda N, Kupiec A, Malina D, Yathirajan HS, Keerthi VR, Chandrasekar N, Dinkar S, Liny P, Adv. Mater. Lett., 4, 332 (2013)

[17] Elavazhagan T, Arunachalam KD, Int. J. Nanomed., 6, 1265 (2011)

[18] Rajesh WK, Jaya RL, Niranjan SK, Vijay DM, Sahebrao BK, Curr. Nanosci., 5, 117 (2009)

[19] Ankamwar B, Damle C, Ahmed A, Sastry M, J. Nanosci. Nanotechnol., 5, 1665 (2005)

[20] Sivakumar P, Nethradevi C, Renganathan S, Asian J. Pharm. Clin. Red., 5(4), 97 (2012)

[21] Poornima K, Gopalakrishnan VK, Chin. J. Biology, 1(2), 1 (2012)