Single chamber membrane-free microbial electrolysis cell (MEC) was operated for the assessment of exoelectrogenic bacteria (EB) growth at carbon felt anode and resultant hydrogen (H-2) production at abiotic cathodes, made using cold rolling (VITO-CoRETM) and casting (VITO-CASE(TM)) methods. Progressive enrichment of EB was observed on anode during 70 days of operation at an applied potential of +0.2 V vs Ag/AgCl, and a maximum current density (CD) of 330.59 mA/m(2) (1.38 mA) was recorded. H-2 production at selected abiotic cathodes was observed, when the enriched bioanode was coupled to them in galvanostat mode between 0.1 and 1.0 mA current range for 10 min each. Higher H-2 production of 114.46 +/- 3.75 mL/m(2) was documented with VITO-CoRETM at 0.6 mA, while 102.76 +/- 3.75 mL/m(2) was recorded with VITO-CASE(TM) at 0.8 mA of current application. This study demonstrates the feasibility of H-2 production on abiotic cathodes using enriched bioanode at low current densities. (C) 2015 Elsevier Ltd. All rights reserved.