This work presents Cu-II(Sal-Ala) complex immobilized on the CuAlLDH as a novel efficient catalyst for artificial superoxide dismutase (SOD) enzyme activity and cyclohexene oxidation. The physico-chemical properties of Cu-II(SalAla)/CuAlLDH were investigated by XRD, XPS, FTIR, DRUV and TGA techniques. The correlation between the catalytic performances, structure and composition of the hybrid catalyst is also discussed.