Quantification of the Mechanical Stress Exerted by Pumps on Mammalian Cells Using anEmulsion as a Non-Biological Model System

Fries T; Dittler I; Blaschczok K; Loffelholz C; Dornfeld W; Schob R; Drews A; Eibl D

Chemie Ingenieur Technik, Vol.88, No.1-2, 177-182, 2016

DOI10.1002/cite.201500126 Export Citation

Quantification of the Mechanical Stress Exerted by Pumps on Mammalian Cells Using anEmulsion as a Non-Biological Model System

Fries T, Dittler I, Blaschczok K, Loffelholz C, Dornfeld W, Schob R, Drews A, Eibl D

The shear stress in the magnetically levitated, bearingless centrifugal pump PuraLev (R) 200SU was determined using the Sauter mean diameter. The data was compared to CHO cell death rates at similar operating parameters. It was shown that besides the specific power input other parameters like the impeller tip speed influence the particle stress in biological systems.

Keywords:Cell death rate;CHO cells;Emulsion;Mass-specific power input;Sauter mean diameter;Shear stress