화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.40, No.2, 181-187, March, 2016
DOI10.7317/pk.2016.40.2.181  Export Citation
조직공학적 인공각막내피를 위한 실크필름의 정제시간이 각막내피세포의 성장에 미치는 영향
Effect of Degumming Time of Silk Films on Growth of Corneal Endothelial Cells for Tissue Engineered Endothelialized Neo-Corneas
이선의, 차세롬, 장나금, 김수영, 김은영, 송정은, 박찬흠1, 강길선†
  • 전북대학교 BIN융합공학과, 고분자나노공학과, 고분자융합소재연구소
  • 1한림대학교 의과대학 춘천성심병원 이비인후과
Seon Eui Lee, Se Rom Cha, Na Keum Jang, Su Young Kim, Eun Young Kim, Jeong Eun Song, Chan Hum Park1, and Gilson Khang†
  • Dept. of BIN Fusion Tech & Dept of PolymerNanoSci Tech, Chonbuk National University, 567 Baekje-daero, Jeonju 54898, Korea
  • 1Dept. of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, 1-1 Okcheon, Chuncheon, Gangwon 24253, Korea
E-mail:
초록
실크는 생분해성 천연고분자로써 이식 후 낮은 면역 거부반응을 일으킨다. 뿐만 아니라, 실크는 다양한 형태로 제조할 수 있으며 제조 방법에 따라 수 마이크로 미터 두께로 조정될 수 있다. 필름 형태의 실크 박막은 매우 투명하며, 높은 수분 및 산소 투과도를 나타낸다. 그러나 실크 단백질인 세리신에 의해 염증반응이 일어난다는 보고가 있으며, 이식 후 분해에 장시간을 필요로 한다. 이 연구에서는 생체 적합성 및 염증반응 등에 세리신 정제시간의 영향을 알기 위해 다양한 정제시간(0, 20, 40, 60분)을 가진 실크로 필름을 제조한 후 접촉각과 세포 생존율 및 RTPCR을 이용한 염증인자발현 실험을 시행하였다. 정제시간에 따라 세리신 함량을 달리한 실크 필름에 각막내피세포파종 후 필름에서의 세포 부착도와 증식률, 특정 mRNA의 발현을 평가하였다. 그 결과 실크의 정제시간은 각막내피세포의 성장에 영향을 주었으며 실크필름의 생명공학 신 각막으로써의 가능성을 보여주었다.
Silk is a biodegradable natural polymer with low immunological rejection after transplantation. As well, silk can be manufactured in various forms and adjusted to the thickness of several micrometers depending on the manufacturing method. Silk shows high transparency in the form of film, high water and oxygen permeability. However, studies have cited some drawbacks of silk, such as inflammatory response due to presence of silk protein (sericin) and its another disadvantage of silk requires a long period of time to decomposition after transplantation. In this study, silk films with various degumming time (0, 20, 40 and 60 min) were fabricated in order to evaluate the effect of sericin in learns of biocompatibility, inflammatory responses, etc. The experiments such as the contact angle, cell viability and inflammatory factor expression using RT-PCR were performed. Corneal endothelial cells were seeded on silk films and examined to evaluate the degree of adhesion in films, cell’s proliferation and specific mRNA expression. This study showed degumming time of silk film fabrication which is a factor for growth of corneal endothelial cells.
Keywords:silk;sericine;film;degumming time;corneal endothelial cells
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