Ni-Fe/Cu/Co/Cu multilayered nanowire arrays were electrodeposited into anodic aluminum oxide template by using dual-bath method at room temperature. Scanning electron microscopy and transmission electron microscopy were used to characterize the morphology and structure of the multilayered nanowire arrays. Vibrating sample magnetometer and physical property measurement system were used to measure their magnetic and giant magnetoresistance (GMR) properties. The effect of sub-layer thickness on the magnetic and GMR properties was investigated. The results indicate that magnetic properties of electrodeposited nanowires are not affected obviously by Cu layer thickness, while magnetic layers (Ni-Fe and Co layers) have significant influence. In addition, GMR ratio presents an oscillatory behavior as Cu layer thickness changes. The magnetic and GMR properties of the multilayered nanowire arrays are optimum at room temperature for the material structure of Ni-Fe (25 nm)/Cu (15 nm)/Co (25 nm)/Cu (15 nm) with 30 deposition cycles. (C) 2015 The Chemical Industry and Engineering Society of China, and Chemical Industry Press. All rights reserved.