The effects of different carbon sources (sugars) on the production and molecular properties of exopolysaccharides (EPS) were evaluated in the mycelial liquid culture of a medicinal fungus Cordyceps sinensis Cs-HK1. Galactose or mannose was used (at 5 g.L-1) as a secondary carbon source with glucose (35g.L-1) at the mass ratio of 1:7. Mannose was consumed notably since the first day of culture, but galactose was not even after glucose was exhausted. The volumetric yield of EPS in culture was increased slightly with the addition of galactose and decreased with mannose. The monosaccharide composition of EPS was also different, e.g., on day 8, the glucose contents of EPS were 76% with the addition of mannose, 59% with galactose, compared with 62% with glucose only. The molecular weight distribution of EPS was also affected by the secondary carbon source, being generally lower compared with that with glucose only. The results suggested that the addition of galactose improved the total yield of EPS in culture while mannose can improve the yield of glucan constituent of EPS. (C) 2015 The Chemical Industry and Engineering Society of China, and Chemical Industry Press. All rights reserved.