A simple electrodeposition approach has been described for the preparation of copper nanoparticles (CuNPs) using biopolymer pectin as a scaffold and graphene as a support. The formation of graphene/pectin-CuNPs was confirmed by scanning electron microscopy, UV-Visible spectroscopy and X-ray diffraction studies. The graphene/pectin-CuNPs film modified electrode was prepared and its electrocatalytic applications to the oxidation of glucose and reduction of H2O2 have been explored. An amperometric glucose sensor was fabricated which exhibited excellent sensor performance in terms of wide linear range (10 mu M-5.5 mM), low detection limit (2.1 mu M) and high sensitivity (0.0457 mu A mu M-1 cm(-2)). Likewise, an amperometric sensor has been fabricated for the determination of H2O2 which displayed linear range of 1 mu M-1 mM, detection limit of 0.35 mu M and sensitivity of 0.391 mu A mu M(-1)cm(-2). The sensor displayed appreciable repeatability, reproducibity and stability. Furthermore, practical feasibility of the sensor has been demonstrated in human serum and contact lens cleaning solution to determine glucose and H2O2, respectively. The main advantages of sensor include simple and green fabrication approach, roughed and stable electrode matrix, high sensitivity and stability, fast in sensing and highly reproducible. (C) 2015 Elsevier Ltd. All rights reserved.