A novel imprinted electrochemical sensor was prepared based on cobalt-nickel bimetallic nanoparticles by one-step electrodeposited on graphene modified carbon electrode for the sensitive determination of octylphenol. Graphene nanosheets were firstly deposited onto carbon electrode surface by electrochemistry reduction method. Then the cobalt-nickel bimetallic nanoparticles were electrodeposited on the graphene modification electrode surface. The electrochemical behaviors of the imprinted sensor were evaluated with cyclic voltammetry and electrochemical impedance spectroscopy in detail. The results showed that the prepared imprinted sensor displayed high sensitivity toward octylphenol attributing to the synergetic effect derived from the cobalt-nickel bimetallic nanoparticles and graphene. Under the optimized condition, the response currents of the imprinted sensor exhibited a linear relationship toward the negative logarithm of concentrations (C-[OP]) ranged from 1.0 x 10(-10) to 1.0 x 10(-7) mol L-1 (R = 0.998) with the detection limit of 3.6 x 10(-11) mol L-1 (S/N = 3). The imprinted electrochemical sensor was applied for the determination of octylphenol in plastic bottles, metal bottles and food packaging bags successfully. (C) 2015 Elsevier Ltd. All rights reserved.