The aim of the current study was to optimise and validate the methodology for determination of gamma-hydroxybutyric acid (GHB) in saliva by CE combined with a contactless conductivity detector ((CD)-D-4) and indirect UV absorbance detection (lambda(ABS) = 210 nm). The optimized BGE, consisting of 8.5 mM maleic acid, 17 mM arginine, 255 mu M cetyltrimethylammonium bromide (CTAB), and 15% acetonitrile, was evaluated for the separation of GHB in saliva within 6 min. The performance characteristics of the CE-(CD)-D-4-indirect UV methodology was validated. The instrument detection and quantification limits were 0.49 and 1.6 mg/L for (CD)-D-4, and 5.1 mg/L and 17.0 mg/L for indirect UV, respectively. The linearity was obtained over the range from 2.5 to 400 mg/L for (CD)-D-4 and from 12.5 to 400 mg/L for indirect UV. The interday precisions were within 2.3-5.7% and intraday precisions were within 1.6-9.0% for (CD)-D-4 as well as 2.1-9.3%, 5.6-10.1% for indirect UV in spiked saliva, respectively. The recoveries were within 87.2-104.4%. The matrix effects were +53.2% for small concentrations up to 25 mg/L for (CD)-D-4 and +23.6% for concentrations up to 75 for mg/L for indirect UV detection. No matrix effects were observed for higher concentration levels. In conclusion, CE-(CD)-D-4-indirect UV can offer a rapid, accurate, sensitive, and definitive method for the determination of GHB abuse in saliva samples as a forensic screening tool.