The widespread use of antibiotics in medicine and as growth-promoting agents has increased the demand for suitable analytical techniques for their analysis. Analytical methods based on CE or miniaturized CE systems have proved over the years their ability for the analysis of antibiotics. Since our last review (Electrophoresis 2014, 35, 28-49) several new CE methodologies have been reported for antibiotic analysis. This review presents an update of the literature published from June 2013 to June 2015 for the analysis of antibiotics by CE. UV continues being the most used detection system for antibiotics analysis by CE. Strategies to improve sensitivity as the use of sensitive detection systems and the application of preconcentration techniques appear to be the major developments. Furthermore, the use of portable and miniaturized devices for antibiotic analysis is presented in detail. Applications of the developed methodologies to the determination of residues of antibiotics in biological, food, and environmental samples are carefully described. Finally, new developments and applications of antibiotics as chiral selectors in CE are also included.